Application
Western blot
Sample
Pig Cell lysate - other (Porcine kidney epithelial cells (PK-15))
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine)
Loading amount
10 µg
Treatment
100 μg/ml poly(I:C) for 18 hrs
Specification
Porcine kidney epithelial cells (PK-15)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk solution (0.1% Tween-20)
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG HRP Conjugate (W401B, Promega)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 15 kDa Non-specific: 17, 40, 180, 200 kDa
Positive control
Mitochondrial fractions.
Additional data
Additional Notes
Ladder: RPN800E (Sigma)
Lane 1: PK-15 (1) cytosolic fraction (untreated)
Lane 2: PK-15 (1) cytosolic fraction (poly(I:C) 100 ´g/ml 18 hrs)
Lane 3: PK-15 (2) cytosolic fraction (untreated)
Lane 4: PK-15 (2) cytosolic fraction (poly(I:C) 100 ´g/ml 18 hrs)
Lane 5: PK-15 (1) mitochondrial fraction (untreated)
Lane 6: PK-15 (1) mitochondrial fraction (poly(I:C) 100 ´g/ml 18 hrs)
Lane 7: PK-15 (2) mitochondrial fraction (untreated)
Lane 8: PK-15 (2) mitochondrial fraction (poly(I:C) 100 ´g/ml 18 hrs)
Membrane: Amersham Protran 0.45 ´m NC
Lysis buffer: Laemmli (in-house)
Protease inhibitors: From kit (ab65311)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Sam Hardy
Verified customer
提交于 Jan 16 2017