HRP Anti-COX IV抗体[mAbcam33985] - Mitochondrial Marker (ab62164)
Key features and details
- HRP Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Conjugation: HRP
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
HRP Anti-COX IV抗体[mAbcam33985] - Mitochondrial Marker
参阅全部 COX IV 一抗 -
描述
HRP小鼠单克隆抗体[mAbcam33985] to COX IV - Mitochondrial Marker -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Xenopus laevis, Chimpanzee, Zebrafish -
免疫原
Synthetic peptide corresponding to Human COX IV aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab16381) -
阳性对照
- WB: HeLa, HEK293, A431, Jurkat and HepG2 whole cell lysates and mouse skeletal muscle tissue lysate. IHC-P: Normal human colon tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
IgG fraction -
克隆
单克隆 -
克隆编号
mAbcam33985 -
骨髓瘤
Sp2/0 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Recombinant Protein
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
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- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62164于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Predicted molecular weight: 15 kDa.
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|
IHC-P |
1/100.
|
说明 |
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WB
1/5000. Predicted molecular weight: 15 kDa. |
IHC-P
1/100. |
靶标
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功能
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the cytochrome c oxidase IV family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 454391 Chimpanzee
- Entrez Gene: 1327 Human
- Entrez Gene: 12857 Mouse
- Entrez Gene: 29445 Rat
- Entrez Gene: 326975 Zebrafish
- Omim: 123864 Human
- SwissProt: O46577 Chimpanzee
- SwissProt: P00423 Cow
see all -
别名
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
see all
图片
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IHC image of COX IV staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab62164 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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All lanes : HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab62164 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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All lanes : HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164) at 1/4000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab62164 被引用在 5 文献中.
- Lobo-Jarne T et al. Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes. EMBO J N/A:e103912 (2020). PubMed: 32511785
- Lowe MM et al. Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues. JCI Insight 4:N/A (2019). PubMed: 31852848
- Serricchio M et al. Cardiolipin synthesizing enzymes form a complex that interacts with cardiolipin-dependent membrane organizing proteins. Biochim Biophys Acta 1863:447-457 (2018). PubMed: 29343430
- Di K et al. Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma. Oncotarget 7:77457-77467 (2016). WB ; Human . PubMed: 27764809
- Powell KA et al. Mitochondrial localization and ocular expression of mutant Opa3 in a mouse model of 3-methylglutaconicaciduria type III. Invest Ophthalmol Vis Sci 52:4369-80 (2011). PubMed: 21613372