Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF-10A, human mammary epithelial cell line)
Specification
MCF-10A, human mammary epithelial cell line
Fixative
Methanol
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse secondary antibody (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
fixation in methanol/acetone 1:1 at -20C for 5 min
Image legend: blue = DAPI and green = COX IV.
Image legend: blue = DAPI and green = COX IV.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 12 2007