Application
Western blot
Sample
Sheep Cell lysate - other (preadipocyte mitochondrial isolates v. cytoplasmic)
Gel Running Conditions
Reduced Denaturing (10% PAG)
Loading amount
10 µg
Specification
preadipocyte mitochondrial isolates v. cytoplasmic
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk/TBS
Dilution
1/800
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit HRP conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
cytoplasmic isolates, lane 3/4
Exposure
20 second(s)
Bands
Specific: 17 kDa
Positive control
mitochondrial isolates, lane 1/2
Additional data
Additional Notes
mitochondrial isolates, lane 1/2
cytoplasmic isolates, lane 3/4
Partial lysate retained in loading well
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Barbara Makela
Verified customer
提交于 Oct 13 2016