Application
Western blot
Sample
Mouse Cell lysate - other (NIH/3T3 cells)
Specification
NIH/3T3 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2 %
Other product details
Dilution
1/5000
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 16 kDa
Positive control
Cells were fractionated into different fractions. Only in he cytoplasmic fraction (1) and not in the nuclear fraction (2) could a band be detected when this antibody was used.
Additional data
Additional Notes
The antibody gave one band in cell lysates form NIH/3T3 cells. The blot was clear and easily detectable.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Camilla Skjerpen
Verified customer
提交于 Jul 04 2005