概述

  • 产品名称
    Anti-CoREST2 / RCOR2抗体
    参阅全部 CoREST2 / RCOR2 一抗
  • 描述
    兔多克隆抗体to CoREST2 / RCOR2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to C terminal amino acids 277-326 (PPEANTKFNS RWTTDEQLLA VQAIRRYGKD FGAIAEVIGN KTLTQVKTF) of Mouse CoREST2/ RCOR2 (NP_473389).

  • 阳性对照
    • Mouse small intestine lysate

性能

应用

Our Abpromise guarantee covers the use of ab113826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-CoREST2 / RCOR2 antibody (ab113826) at 1 µg/ml + Mouse small intestine lysate at 10 µg

    Predicted band size: 58 kDa



    Gel concentration: 12%

实验方案

文献

This product has been referenced in:
  • Hiramoto M  et al. A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression. Int J Mol Med 41:717-728 (2018). WB ; Rat . Read more (PubMed: 29207083) »

See 1 Publication for this product

客户评价及客户问答

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Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (HEK293T overexpressing Flag-tagged mouse RCOR2)
Total protein in input
600 µg
Immuno-precipitation step
Other - Prot G Dynabeads
Specification
HEK293T overexpressing Flag-tagged mouse RCOR2
Username

Abcam user community

Verified customer

提交于 Nov 24 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293T overexpressing Flag-tagged mouse RCOR2 and)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
60 µg
Specification
HEK293T overexpressing Flag-tagged mouse RCOR2 and
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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