Anti-COPB2抗体(ab2915)

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Western blot - Anti-COPB2 antibody (ab2915)

    Key features and details

    • Rabbit polyclonal to COPB2
    • Suitable for: WB
    • Reacts with: Rat, Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-COPB2抗体
      参阅全部 COPB2 一抗

    • 描述

      兔多克隆抗体to COPB2

    • 宿主

      Rabbit

    • 特异性

      Detects coatomer-protein I beta (COP I beta).

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Rat, Human
      预测可用于: Mouse, Cow, Cynomolgus monkey

    • 免疫原

      Synthetic peptide corresponding to Rat COPB2 aa 494-511.
      Sequence:

      KVLAAQETHEGVTEDGIE


      (Peptide available as ab4933)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

       This product was previously labelled as beta COP I

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • Primary antibody说明

      Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab2915 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use a concentration of 2 µg/ml. Predicted molecular weight: 115 kDa.

    靶标

    • 功能

      The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
      This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner.

    • 序列相似性

      Belongs to the WD repeat COPB2 family.
      Contains 9 WD repeats.

    • 细胞定位

      Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • Beta'-coat protein antibody
      • Beta'-COP antibody
      • Coatomer subunit beta' antibody
      • Copb2 antibody
      • COPB2_HUMAN antibody
      • p102 antibody
      see all

    图片

    • Western blot - Anti-COPB2 antibody (ab2915)
      Western blot - Anti-COPB2 antibody (ab2915)
      Western blot detection of beta COP I from PC-12 cell lysates using ab2915.

    文献 (0)

    发表研究结果有使用 ab2915？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2915 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    I would like to know how long this product has been available and have you had any comments from users. I have tried this antibody from another company and it did not work in my system. I am using a concentrated Chinese hamster ovary cell cytosol prep in an immunoprecipitation system and I want to use the anti- B' COP antibody for a Western after the IP. Do you think your antibody would work and could I possibly get credit on an other antibody if it did not. Thanks

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    Answer

    Thank you for your enquiry and your interest in our products. All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). This antibody has only been tested and characterised for Western blot application and it has not been tested for IP. However, I have conducted a search for you on our website and actually we have another antibody in stock AB6324:Mouse monoclonal (M3A5) to beta-COP. This product is suitable for IP and Western blot application, please take a look at the datasheet you may find some useful information.

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