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Neuroscience Neurotransmitter Biogenic Amines Noradrenaline

Anti-Conjugated Noradrenaline抗体(ab6454)

  • Datasheet
Submit a review Submit a question References (2)

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Key features and details

  • Rabbit polyclonal to Conjugated Noradrenaline
  • Suitable for: ELISA, IHC-Fr
  • Reacts with: Species independent
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Conjugated Noradrenaline抗体
  • 描述

    兔多克隆抗体to Conjugated Noradrenaline
  • 宿主

    Rabbit
  • 特异性

    Using catecholamine-Glutaraldehyde-BSA conjugates, antibody specificity was performed with an ELISA test by competition experiments, as follows: (a) unconjugated or conjugated catecholamine concentration at half displacement (b) Non-reduced conjugate
    CompoundCross-reactivity ratio (a)
    Noradrenaline1
    Octopamine1/31
    Dopamine1/70
    Adrenaline1/177
    Noradrenaline (b)1/316
    L-DOPA, Tyramine, unconjugated Noradrenaline1/>5000
  • 经测试应用

    适用于: ELISA, IHC-Frmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Synthetic noradrenaline conjugated to protein carriers (PC).

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • 纯度

    Ammonium Sulphate Precipitation
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Signal Transduction
    • Growth Factors/Hormones
    • Adrenalin

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

Our Abpromise guarantee covers the use of ab6454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
IHC-Fr
  • 应用说明
    Immunohistochemistry: 1/2000 - 1/5000. Tested using a PAP techique on free floating rat locus coeruleus.
    ELISA: assay dependent.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.
  • 靶标

    • 相关性

      Noradrenaline (NA) is a catecholamine neurotransmitter at postganglionic sympathetic fibres, produced following a series of steps from the essential amino acid phenylalanine. Terminal postganglionic sympathetic fibres have varicosities (where NA is synthesised and stored) which form synapses with the effector organ. The adrenal medulla is a major source of catecholamines (such as NA and adrenaline), however, most of the NA produced in the adrenal medulla is converted into adrenaline. NA is key in the fight-or-flight response and also directly increases heart rate, energy release from fat, and muscle readiness.

    实验方案

    • Immunohisto and cytochemistry

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (2)

    发表研究结果有使用 ab6454?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6454 被引用在 2 文献中.

    • Aschrafi A  et al. Angiotensin II mediates the axonal trafficking of tyrosine hydroxylase and dopamine ß-hydroxylase mRNAs and enhances norepinephrine synthesis in primary sympathetic neurons. J Neurochem 150:666-677 (2019). PubMed: 31306490
    • Arima Y  et al. Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit. Elife 6:N/A (2017). PubMed: 28809157

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