重组Anti-COMT抗体[EPR6490] (ab126618)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6490] to COMT
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-COMT抗体[EPR6490]
参阅全部 COMT 一抗 -
描述
兔单克隆抗体[EPR6490] to COMT -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human COMT. Human COMT is a soluble isoform.
Database link: P21964 -
阳性对照
- WB: HEK-293T, HAP1, A431, MOLT4 and MCF7 cell lysates; Human fetal liver lysate. IHC-P: Human liver and kidney tissues.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6490 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126618于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
|
说明 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa). |
靶标
-
功能
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. -
组织特异性
Brain, liver, placenta, lymphocytes and erythrocytes. -
序列相似性
Belongs to the mammalian catechol-O-methyltransferase family. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Cytoplasm and Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 1312 Human
- Entrez Gene: 12846 Mouse
- Entrez Gene: 24267 Rat
- Omim: 116790 Human
- SwissProt: P21964 Human
- SwissProt: O88587 Mouse
- SwissProt: P22734 Rat
- Unigene: 370408 Human
see all -
别名
- Catechol O methyltransferase antibody
- Catechol O-methyltransferase antibody
- COMT antibody
see all
图片
-
All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution (Purified)
Lane 1 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Rat spleen lysate
Lane 4 : Rat kidney lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 30 kDa -
All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : COMT knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 24-28 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab126618 observed at 24-28 kDa. Red - loading control ab8245 observed at 37 kDa.
ab126618 Anti-COMT antibody [EPR6490] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266537 (knockout cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab126618 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution
Lane 1 : Wild type HAP1 whole cell lysate at 40 µg
Lane 2 : COMT knockout HAP1 whole cell lysate at 40 µg
Lane 3 : A431 whole cell lysate at 20 µg
Lane 4 : MCF7 whole cell lysate at 20 µg
Predicted band size: 30 kDaLanes 1 - 4: Merged signal (red and green). Green - ab126618 observed at 24/28 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab126618 was shown to specifically react with COMT when COMT knockout samples were used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. Samples were incubated with ab126618 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at 1/1000 and 1/10000 dilutions respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab126618 被引用在 6 文献中.
- Ng J et al. Gene therapy restores dopamine transporter expression and ameliorates pathology in iPSC and mouse models of infantile parkinsonism. Sci Transl Med 13:N/A (2021). PubMed: 34011628
- Cooper TK et al. Coati Bodies: Cytoplasmic Hyaline Globules in the Ganglionic Neurons of Aging Captive Coatis. Vet Pathol 54:851-854 (2017). PubMed: 28578627
- Winner BM et al. Metabolism of Dopamine in Nucleus Accumbens Astrocytes Is Preserved in Aged Mice Exposed to MPTP. Front Aging Neurosci 9:410 (2017). PubMed: 29311899
- Doan KV et al. FoxO1 in dopaminergic neurons regulates energy homeostasis and targets tyrosine hydroxylase. Nat Commun 7:12733 (2016). PubMed: 27681312
- Stefanovic B et al. Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. Eur Neuropsychopharmacol 26:1629-37 (2016). WB ; Rat . PubMed: 27499503
- Wakuda T et al. Perinatal asphyxia alters neuregulin-1 and COMT gene expression in the medial prefrontal cortex in rats. Prog Neuropsychopharmacol Biol Psychiatry 56:149-54 (2015). PubMed: 25194460