Anti-Collagen VII抗体[LH7.2] (ab6312)

Reviews (3)Q&A (5)References (5)
Immunohistochemistry (Frozen sections) - Anti-Collagen VII antibody [LH7.2] (ab6312)

    Key features and details

    • Mouse monoclonal [LH7.2] to Collagen VII
    • Suitable for: IHC-Fr
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-Collagen VII抗体[LH7.2]
      参阅全部 Collagen VII 一抗

    • 描述

      小鼠单克隆抗体[LH7.2] to Collagen VII

    • 宿主

      Mouse

    • 经测试应用

      适用于: IHC-Frmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Full length native protein (purified) corresponding to Human Collagen VII.

    • 表位

      Recognizes an epitope located on collagenase digested Type VII collagen (150kD), i.e. the non-helical carboxy terminal region of the Type VII collagen dimer. Immunoelectron microscopy shows antibody binding localised at the inferior border of the lamina densa.

    • 阳性对照

      • IHC-Fr: Human tonsil.

    • 常规说明

      This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR158908. Lot numbers higher than GR158908 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.”

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 纯度

      Proprietary Purification

    • 纯化说明

      Purified from Tissue culture supernatant.

    • 克隆

      单克隆

    • 克隆编号

      LH7.2

    • 同种型

      IgG1

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6312于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-Fr (2)
    Use a concentration of 1.5 - 3 µg/ml.
    说明
    IHC-Fr
    Use a concentration of 1.5 - 3 µg/ml.

    靶标

    • 功能

      Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

    • 疾病相关

      Epidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
      Epidermolysis bullosa dystrophica, autosomal dominant
      Epidermolysis bullosa dystrophica, autosomal recessive
      Epidermolysis bullosa dystrophica, Pasini type
      Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
      Transient bullous dermolysis of the newborn
      Epidermolysis bullosa dystrophica, pretibial type
      Epidermolysis bullosa dystrophica, Bart type
      Epidermolysis bullosa pruriginosa
      Nail disorder, non-syndromic congenital, 8
      Epidermolysis bullosa dystrophica, with subcorneal cleavage

    • 序列相似性

      Contains 1 BPTI/Kunitz inhibitor domain.
      Contains 9 fibronectin type-III domains.
      Contains 2 VWFA domains.

    • 翻译后修饰

      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

    • 细胞定位

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Alpha 1 type VII collagen antibody
        • CO7A1_HUMAN antibody
        • Col7a1 antibody
        • Collagen 7 antibody
        • Collagen alpha 1(VII) chain antibody
        • Collagen alpha-1(VII) chain antibody
        • Collagen type VII alpha 1 antibody
        • Collagen VII alpha 1 polypeptide antibody
        • CollagenVII antibody
        • EBD 1 antibody
        • EBD1 antibody
        • EBDCT antibody
        • EBR 1 antibody
        • EBR1 antibody
        • LC collagen antibody
        • Long chain collagen antibody
        • Long-chain collagen antibody
        see all

      图片

      • Immunohistochemistry (Frozen sections) - Anti-Collagen VII antibody [LH7.2] (ab6312)
        Immunohistochemistry (Frozen sections) - Anti-Collagen VII antibody [LH7.2] (ab6312)
        Immunohistochemistry analysis of frozen acetone human tonsil sections labeling Collagen VII with ab6312 at 3 μg/mL followed by FITC-Anti-Mouse IgG.
         

         

      文献 (5)

      发表研究结果有使用 ab6312？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6312 被引用在 5 文献中.

      • Derr K  et al. Fully Three-Dimensional Bioprinted Skin Equivalent Constructs with Validated Morphology and Barrier Function. Tissue Eng Part C Methods 25:334-343 (2019). PubMed: 31007132
      • Bertram KM  et al. Identification of HIV transmitting CD11c+ human epidermal dendritic cells. Nat Commun 10:2759 (2019). PubMed: 31227717
      • Bage T  et al. Ex vivo culture of keratinocytes on papillary and reticular dermal layers remodels skin explants differently: towards improved wound care. Arch Dermatol Res 311:647-652 (2019). PubMed: 31168656
      • Benny P  et al. Making more matrix: enhancing the deposition of dermal-epidermal junction components in vitro and accelerating organotypic skin culture development, using macromolecular crowding. Tissue Eng Part A 21:183-92 (2015). PubMed: 25058150
      • Ponsioen TL  et al. Human retinal Müller cells synthesize collagens of the vitreous and vitreoretinal interface in vitro. Mol Vis 14:652-60 (2008). ICC/IF ; Human . PubMed: 18385800

      客户评价及客户问答

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      1-8 of 8 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-Collagen VII antibody [LH7.2]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (HUman skin (foreskin))
      Permeabilization
      No
      Specification
      HUman skin (foreskin)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Fixative
      Unfixed
      Read More

      Abcam user community

      Verified customer

      提交于 Mar 12 2020

      Immunohistochemistry (Frozen sections) abreview for Anti-Collagen VII antibody [LH7.2]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Skin)
      Permeabilization
      No
      Specification
      Skin
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      提交于 Jul 11 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen VII antibody [LH7.2]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
      Antigen retrieval step
      Enzymatic - Buffer/Enzyme Used: Proteinase K
      Sample
      Human Tissue sections (Skin)
      Specification
      Skin
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

      Mr. Carl Hobbs

      Verified customer

      提交于 Aug 19 2014

      Question

      Do these antibodies need to be run under denaturing or on denaturing conditions?

      Read More
      Answer

      Thank you for contacting us.
      Both antibodies were run under reducing and denaturing conditions.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      WB with conditioned medium from fibroblasts and lysates (human)
      send reference

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      Answer

      Thank you for contacting us.

      I have attached the reference paper which the lab cited as researchers having used this antibody.
      As far as I could see they did do the collagenase treatment and obtained the 150 kDa band. This is most likely the source where the information on the datasheet comes from.

      Let me know if I shall ask the lab additional information in this regard.

      I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      I would like to test ab6312 and ab80790 in mouse via IHC.

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      Answer



      I am very pleased to hear you would like to accept our offer and test ab6312 and ab80790in mouse. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF THE PRODUCT OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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      Question

      We have bought several antibodies against human collagens: ab6307, ab7778, ab292, ab6311, ab6312, ab6583, ab7045. We performed our first Western Blot and now we want to know which band corresponds with a certain collagen. Sometimes (eg collagen IV), we saw several lanes. I hope you can give us the sizes of the lanes, which become visible after using your antibodies. In advance, I would thank you for your time. Dirk Ponsioen

      Read More
      Answer

      Thank you very much for your enquiry. All the information that we have regarding these antibodies is located on the online datasheets, please refer to them for more information. Here are the expected band sizes for these antibodies: ab7045 - 21.6 kDa ab7778 - Collagen III has a major band at 150kDa under reducing conditions. ab292 - The M.Wt of collagen Type I is at least 95kDa. ab6312 - Recognizes an epitope located on collagenase digested Type VII collagen (150kD), i.e. the non-helical carboxy terminal region of the Type VII collagen dimer. ab6307 - 70 kDa I will get back to you regarding ab6583 and ab6311. Let me know if you have any more questions.

      Read More

      Question

      Please let me know the source of recombinant human type VII collagen. Thank you. With kind regards, Mohammed S. Razzaque

      Read More
      Answer

      The immunogen used to raise this antibody was insoluble fractions prepared from neonatal foreskin epidermal cells. Unfortunately, I have not been able to source a supplier for the recombinant protein.

      Read More

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