Key features and details
- Rabbit polyclonal to Collagen VI
- Suitable for: WB
- Reacts with: Cow, Human
- Isotype: IgG
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
参阅全部 Collagen VI 一抗
描述兔多克隆抗体to Collagen VI
特异性Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
经测试应用适用于: WBmore details
种属反应性与反应: Cow, Human
预测可用于: Mouse, Rat, Sheep, Rabbit, Dog, Pig, Mammals
Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028. Collagen Type VI from human and bovine placenta.
Database link: P12109
- WB: HepG2 whole cell lysate.
At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.44% Sodium chloride, 4.8% Sodium borate, 0.15% EDTA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Our Abpromise guarantee covers the use of ab6588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa).
This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.
功能Collagen VI acts as a cell-binding protein.
疾病相关Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
序列相似性Belongs to the type VI collagen family.
Contains 3 VWFA domains.
翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
细胞定位Secreted > extracellular space > extracellular matrix.
- Information by UniProt
- Alpha 1 (VI) chain (61 AA) antibody
- CO6A1_HUMAN antibody
- COL6A1 antibody
Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 108 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
ab6588 被引用在 91 文献中.
- Jones JEC et al. The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet. Sci Rep 10:2380 (2020). PubMed: 32047213
- Harish P et al. Inhibition of Myostatin Reduces Collagen Deposition in a Mouse Model of Oculopharyngeal Muscular Dystrophy (OPMD) With Established Disease. Front Physiol 11:184 (2020). PubMed: 32194441
- Semple C et al. Partial Weight-Bearing in Female Rats: Proof of Concept in a Martian-Gravity Analog. Front Physiol 11:302 (2020). PubMed: 32308630
- Bolduc V et al. Pathogenic variants in COL6A3 cause Ullrich-like congenital muscular dystrophy in young Labrador Retriever dogs. Neuromuscul Disord 30:360-367 (2020). PubMed: 32439203
- Farini A et al. PTX3 Predicts Myocardial Damage and Fibrosis in Duchenne Muscular Dystrophy. Front Physiol 11:403 (2020). PubMed: 32508664
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