Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ovary)
Specification
Ovary
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.4
Other product details
Dilution
1/400
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: From the Vectastain kit (Vector)
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Additional data
Additional Notes
Antigens were retrieved by boiling with an antigen unmasking solution (Vector Laboratories, Burlingame, CA, USA) for 20 min at 120ºC in an autoclave and then cooled down in water for 5 min.
Picture: Blood vessels in whole ovarian section. Clear staining is seen in the vessel walls
Picture: Blood vessels in whole ovarian section. Clear staining is seen in the vessel walls
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Birgitta Weijdegard
Verified customer
提交于 Oct 23 2006