Key features and details
- Mouse monoclonal [COL-94] to Collagen IV
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
参阅全部 Collagen IV 一抗
描述小鼠单克隆抗体[COL-94] to Collagen IV
经测试应用适用于: IHC-Pmore details
预测可用于: Non human primates不与反应: Rat, Sheep, Goat, Chicken, Cat
Full length native protein (purified) corresponding to Human Collagen IV.
Database link: P02462
表位Recognises an epitope located on the alpha 1 and/or alpha 2 chains of human collage type IV.
- Natural Human Collagen IV protein (ab7536) can be used as a positive control in WB. Human skin tissue.
This product was changed from ascites to tissue culture supernatant on 10th July 2019. Lot numbers higher than GR3230927 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium azide
Concentration information loading...
纯化说明Purified from hybridoma cell culture
Our Abpromise guarantee covers the use of ab6311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 8 µg/ml.
The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
功能Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
组织特异性Highly expressed in placenta.
疾病相关Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
序列相似性Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
结构域Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
翻译后修饰Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, arresten.
细胞定位Secreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- Arresten antibody
- BSVD antibody
- CO4A1_HUMAN antibody
Immunohistochemical analysis of paraffin-embedded human tongue tissue staining Collagen IV with ab6311 at 8µg/ml. The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovary tissue sections labeling Collagen IV with unpurified ab6311 at 1/400 dilution. Tissue was blocked with 1.4% serum for 30 minutes. Heat mediated antigen retrieval was performed. An undiluted monoclonal alkaline phosphatase conjugated secondary antibody was used.
Blood vessels in whole ovarian section. Clear staining is seen in the vessel walls.
This image was generated using the ascites version of the product.
ab6311 被引用在 28 文献中.
- Yemanyi F et al. Glucocorticoid-induced cell-derived matrix modulates transforming growth factor ß2 signaling in human trabecular meshwork cells. Sci Rep 10:15641 (2020). PubMed: 32973273
- Kostov K & Blazhev A Use of Glycated Hemoglobin (A1c) as a Biomarker for Vascular Risk in Type 2 Diabetes: Its Relationship with Matrix Metalloproteinases-2, -9 and the Metabolism of Collagen IV and Elastin. Medicina (Kaunas) 56:N/A (2020). PubMed: 32403389
- Petrosyan A et al. A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier. Nat Commun 10:3656 (2019). PubMed: 31409793
- Matsumoto E et al. Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine. PLoS One 14:e0212369 (2019). PubMed: 30865653
- Solé C et al. An Exosomal Urinary miRNA Signature for Early Diagnosis of Renal Fibrosis in Lupus Nephritis. Cells 8:N/A (2019). PubMed: 31349698