Anti-Collagen IV抗体[COL-94] (ab6311)

Reviews (10)Q&A (14)References (26)

Key features and details

  • Mouse monoclonal [COL-94] to Collagen IV
  • Suitable for: IHC-P, Dot blot
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Collagen IV抗体[COL-94]
    参阅全部 Collagen IV 一抗

  • 描述

    小鼠单克隆抗体[COL-94] to Collagen IV

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, Dot blotmore details

  • 种属反应性

    与反应: Human
    预测可用于: Non human primates不与反应: Rat, Sheep, Goat, Chicken, Cat

  • 免疫原

    Full length native protein (purified) corresponding to Human Collagen IV.
    Database link: P02462

  • 表位

    Recognises an epitope located on the alpha 1 and/or alpha 2 chains of human collage type IV.

  • 阳性对照

    • Natural Human Collagen IV protein (ab7536) can be used as a positive control in WB. Human skin tissue.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 10th July 2019. Lot numbers higher than GR3230927 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

     

性能

应用

Our Abpromise guarantee covers the use of ab6311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 8 µg/ml.

The antibody was developed using biotin/ExtrAvidin®-Peroxidase.
Dot blot Use at an assay dependent concentration.

靶标

  • 功能

    Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

  • 组织特异性

    Highly expressed in placenta.

  • 疾病相关

    Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
    Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
    Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.

  • 序列相似性

    Belongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.

  • 结构域

    Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.

  • 翻译后修饰

    Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Proteolytic processing produces the C-terminal NC1 peptide, arresten.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Arresten antibody
    • BSVD antibody
    • CO4A1_HUMAN antibody
    • COL4A1 antibody
    • COL4A1 NC1 domain antibody
    • COL4A2 antibody
    • COL4A3 antibody
    • COL4A4 antibody
    • COL4A5 antibody
    • collagen alpha-1(IV) chain antibody
    • Collagen IV Alpha 1 Polypeptide antibody
    • Collagen IV Alpha 2 Polypeptide antibody
    • Collagen Of Basement Membrane Alpha 1 Chain antibody
    • Collagen Of Basement Membrane Alpha 2 Chain antibody
    • Collagen Type IV Alpha 1 antibody
    • collagen type IV alpha 1 chain antibody
    • Collagen Type IV Alpha 2 antibody
    • Collagen Type IV Alpha 3 antibody
    • Collagen Type IV Alpha 4 antibody
    • Collagen Type IV Alpha 5 antibody
    • RATOR antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)

    Immunohistochemical analysis of paraffin-embedded human tongue tissue staining Collagen IV with ab6311 at 8µg/ml. The antibody was developed using biotin/ExtrAvidin®-Peroxidase.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)This image is courtesy of an abreview submitted by Birgitta Weijdegard, Sahlgrenska Cancer Center.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovary tissue sections labeling Collagen IV with unpurified ab6311 at 1/400 dilution. Tissue was blocked with 1.4% serum for 30 minutes. Heat mediated antigen retrieval was performed. An undiluted monoclonal alkaline phosphatase conjugated secondary antibody was used.
    Blood vessels in whole ovarian section. Clear staining is seen in the vessel walls.

    This image was generated using the ascites version of the product.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen IV antibody [COL-94] (ab6311)
    Immunocytochemistry/ Immunofluorescence - Anti-Collagen IV antibody [COL-94] (ab6311)This image is courtesy of an anonymous Abreview

    Immunofluorescence analysis of Human endothelial cells, staining Collagen IV with unpurified ab6311.
    Cells were fixed with formaldehyde and permeabilized with acetone. Samples were incubated with primary antibody (0.5 µg/ml in diluent) for 18 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (5 µg/ml) (ab150113) was used as the secondary antibody.

    This image was generated using the ascites version of the product.

    See Abreview

文献 (26)

发表研究结果有使用 ab6311？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6311 被引用在 26 文献中.

  • Matsumoto E  et al. Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine. PLoS One 14:e0212369 (2019). PubMed: 30865653
  • Solé C  et al. An Exosomal Urinary miRNA Signature for Early Diagnosis of Renal Fibrosis in Lupus Nephritis. Cells 8:N/A (2019). PubMed: 31349698
  • Hamilton NJI  et al. Using a Three-Dimensional Collagen Matrix to Deliver Respiratory Progenitor Cells to Decellularized Trachea In Vivo. Tissue Eng Part C Methods 25:93-102 (2019). PubMed: 30648458
  • Petrosyan A  et al. A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier. Nat Commun 10:3656 (2019). PubMed: 31409793
  • Peng C  et al. Lysyl oxidase activates cancer stromal cells and promotes gastric cancer progression: quantum dot-based identification of biomarkers in cancer stromal cells. Int J Nanomedicine 13:161-174 (2018). PubMed: 29343955
View all Publications for this product

客户评价及客户问答

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1-10 of 24 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen IV antibody [COL-94]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Mesenchymal tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Mesenchymal tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin
Read More

Abcam user community

Verified customer

提交于 Mar 13 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen IV antibody [COL-94]

 Average
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin
Read More

Abcam user community

Verified customer

提交于 Mar 13 2019

ELISA abreview for Anti-Collagen IV antibody [COL-94]

 Average
Abreviews
abreview image
Application
ELISA
Sample
Human Purified protein (collagen IV)
Specification
collagen IV
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Type
Sandwich (Capture)
Read More

Patricia Carulla

Verified customer

提交于 Aug 17 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen IV antibody [COL-94]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Sep 08 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen IV antibody [COL-94]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.05% typsin
Sample
Human Tissue sections (human whole fat tissue)
Specification
human whole fat tissue
Permeabilization
No
Fixative
Formaldehyde
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Abcam user community

Verified customer

提交于 Mar 18 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen IV antibody [COL-94]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (kidney)
Specification
kidney
Permeabilization
No
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Feb 11 2015

Question

Thanks for your reply. BTW, I am interested in performing IHC on rabbit tissue. That is what we are talking about, correct?
I will check the biocompare website and get back to you if we can not find what we are looking for.
Sounds like a mouse anti-human Ab would be a reasonable guess, right?

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Answer

Thank you for contacting us.

Thank you for clarifying that you would prefer to do IHC on paraffin tissue sections rather than frozen tissue sections. For both of these applications I would indeed suggest checking the biocompare website. Ideally you would need a mouse anti-rabbit collagen IV antibody. If this is not possible, then I would try looking for a mouse anti-human collagen IV that has been proven to react with rabbit collagen IV since unfortunately, we do not have this in our catalogue.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Thank you for your swift reply.
We are interested in your offer and would like to participate if you can find an antibody that might work. we do realize that the restrictions of working with rabbit tissue and paraffinized sections does not make the process easier. The use of frozen tissue sections would be a possibility if that is likely to be the only option that works, but it would not be our preferred choice.
BTW, the Acris antibody was purchased through Labforce.

I unfortunately do not have the protein sequences for rabbit collagen IV. Can try to look those up if any info exists, i am just not sure when I will be able to get to that.
Thanks again for the reply.

Read More
Answer

Thank you for contacting us.

I am sorry, I was under the impression that you wanted to do immunohistochemistry on rabbit tissue. If this is not the case, what application would you like to use the anti-collagen IV antibody in?

With regards to finding an antibody which is likely to work rabbit, I have managed to find the protein sequences of the alpha chain 1,2 and 4 of rabbit collagen IV at:

http://www.uniprot.org/uniprot/Q7M2X2
http://www.uniprot.org/uniprot/Q7M2X5
http://www.uniprot.org/uniprot/P55787

respectively.

However, some of these sequences are incomplete. For the amino acid data we do have, it appears that there is a relatively high % homology between rabbit and human however because, our data is incomplete and it appears that the rabbit protein may be smaller, I am unable to predict whether an antibody is likely to work or not in rabbit.

I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers to see if they have an anti-collagen IV predicted to work in rabbit. The links are:
www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

Otherwise, if you would like to test one of the antibodies from our catalogue we have 7 mouse monoclonals which are guaranteed to work in IHC to choose from. If you would like to test one of them, please contact us letting us know which one you would like to test.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen IV antibody [COL-94]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Endothelial in Matrigel)
Specification
Endothelial in Matrigel
Fixative
Formaldehyde
Permeabilization
Yes - Acetone
Read More

Abcam user community

Verified customer

提交于 Sep 25 2012

Immunohistochemistry (Frozen sections) abreview for Anti-Collagen IV antibody [COL-94]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Trititon X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Jul 17 2012

1-10 of 24 Abreviews or Q&A

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