Anti-Collagen IV抗体(ab19808)
Key features and details
- Rabbit polyclonal to Collagen IV
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Collagen IV抗体
参阅全部 Collagen IV 一抗 -
描述
兔多克隆抗体to Collagen IV -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Full length native protein (extracted and purified from tumor tissues) (Mouse).
-
阳性对照
- IHC-P: Mouse skin tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Ion exchange chromatography (DEAE-Trisacryl). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab19808于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (4) |
1/500.
|
说明 |
---|
IHC-P
1/500. |
靶标
-
功能
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. -
组织特异性
Highly expressed in placenta. -
疾病相关
Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles. -
序列相似性
Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain. -
结构域
Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain. -
翻译后修饰
Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, arresten. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 12826 Mouse
- Entrez Gene: 12827 Mouse
- Entrez Gene: 12828 Mouse
- Entrez Gene: 12829 Mouse
- Entrez Gene: 12830 Mouse
- SwissProt: P02463 Mouse
- SwissProt: P08122 Mouse
- SwissProt: Q9QZR9 Mouse
see all -
别名
- Arresten antibody
- BSVD antibody
- CO4A1_HUMAN antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labeling Collagen IV with ab19808 at ≥1/500 dilution.
-
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for Collagen IV using ab19808.
Endocrine-cells coated with a layer of extracellular matrix (P1). Immunohistochemical staining for Insulin (green), glucagon (red) and collagen IV (yellow) is shown. Note that intra-islet blood vessels are also associated with the extracellular matrix. Scale bar is 50 µm.
The primary antibodies were detected using different combinations of Cy2, Cy5 and Texas Red-conjugated secondary antibodies and the image was captured via confocal microscopy.
-
ab19808 at a 1/200 dilution staining mouse mammary gland tissue by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 16 hours and then detected with an Alexa Fluor® 488 conjuaged anti-rabbit antibody.
数据表及文件
-
SDS download
-
Datasheet download
文献 (158)
ab19808 被引用在 158 文献中.
- Zhang M et al. Asiaticoside inhibits renal fibrosis development by regulating the miR-142-5p/ACTN4 axis. Biotechnol Appl Biochem 69:313-322 (2022). PubMed: 33444480
- Tsujikawa K et al. Developmentally interdependent stretcher-compressor relationship between the embryonic brain and the surrounding scalp in the preosteogenic head. Dev Dyn 251:1107-1122 (2022). PubMed: 34997665
- Shen J et al. TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy. Diabetol Metab Syndr 14:26 (2022). PubMed: 35120573
- Xiao A et al. Sequential and Dynamic Variations of IL-6, CD18, ICAM, TNF-α, and Microstructure in the Early Stage of Diabetic Retinopathy. Dis Markers 2022:1946104 (2022). PubMed: 35126785
- Mostafavi H et al. Interleukin-17 contributes to Ross River virus-induced arthritis and myositis. PLoS Pathog 18:e1010185 (2022). PubMed: 35143591