Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Esophagus tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA Buffer pH 9.0
Permeabilization
No
Specification
Esophagus tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% normal goat serum
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: ImmPRESS* ANTI-RABBIT IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
Abcam has not tested ab7778 in Pig and does not know how it should have performed. This researcher has received a credit for testing ab7778 in Pig
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
LAI Hua Sheng
Verified customer
提交于 Aug 30 2021