Key features and details
- Rabbit polyclonal to COL1A2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
参阅全部 COL1A2 一抗
经测试应用适用于: WB, ICC/IFmore details
预测可用于: Mouse, Cow, Dog, Monkey
Recombinant protein fragment corresponding to a region within amino acids 1057 and 1339 of COL1A2 (NP_000080).
- WB: 293T and A431 cell lysate ICC/IF: A431 cell
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
纯度Immunogen affinity purified
功能Type I collagen is a member of group I collagen (fibrillar forming collagen).
组织特异性Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
疾病相关Ehlers-Danlos syndrome 7B
Osteogenesis imperfecta 1
Osteogenesis imperfecta 2
Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form
Osteogenesis imperfecta 3
Osteogenesis imperfecta 4
A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1.
序列相似性Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
结构域The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
细胞定位Secreted > extracellular space > extracellular matrix.
- Information by UniProt
- Alpha 2 collagen type I antibody
- Alpha 2 type I collagen antibody
- Alpha 2 type I procollagen antibody
All lanes : Anti-COL1A2 antibody (ab96723) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 129 kDa
7.5% SDS PAGE
Immunofluorescence analysis of COL1A2 in paraformaldehyde fixed A431 cell, using ab96723 at a 1/200 dilution.
Lower image: merged with DNA probe.
ab96723 被引用在 37 文献中.
- Liu H et al. Silencing microRNA-29b-3p expression protects human trabecular meshwork cells against oxidative injury via upregulation of RNF138 to activate the ERK pathway. Int J Mol Med 47:N/A (2021). PubMed: 33907817
- Xu J et al. Ubiquitously specific protease 4 inhibitor-Vialinin A attenuates inflammation and fibrosis in S100-induced hepatitis mice through Rheb/mTOR signalling. J Cell Mol Med 25:1140-1150 (2021). PubMed: 33295107
- Hu H et al. Role of microRNA-335 carried by bone marrow mesenchymal stem cells-derived extracellular vesicles in bone fracture recovery. Cell Death Dis 12:156 (2021). PubMed: 33542183
- Huang Q et al. 0.5-Gy X-ray irradiation induces reorganization of cytoskeleton and differentiation of osteoblasts. Mol Med Rep 23:N/A (2021). PubMed: 33760136
- Li L et al. Gold Nanoparticles Combined Human ß-Defensin 3 Gene-Modified Human Periodontal Ligament Cells Alleviate Periodontal Destruction via the p38 MAPK Pathway. Front Bioeng Biotechnol 9:631191 (2021). PubMed: 33585435