概述

  • 产品名称
    CNQX disodium salt
  • 描述
    AMPA / kainate拮抗剂; water soluble
  • 生物学描述
    Water soluble, potent, competitive AMPA / kainate receptor antagonist. Also antagonist at NMDA receptor glycine site.

    Also available in simple stock solutions (ab144488) - add 1 ml of water to get an exact, ready-to-use concentration.
  • 纯度
    > 99%

性能

应用

Our Abpromise guarantee covers the use of ab120044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

图片

  • ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX disodium salt (ab120044), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX disodium salt, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120044 (CNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

文献

This product has been referenced in:
  • Long SK  et al. Effect of 6-cyano-2,3-dihydroxy-7-nitro-quinoxaline (CNQX) on dorsal root-, NMDA-, kainate- and quisqualate-mediated depolarization of rat motoneurones in vitro. Br J Pharmacol 100:850-4 (1990). Read more (PubMed: 1976402) »
  • Honoré T  et al. Quinoxalinediones: potent competitive non-NMDA glutamate receptor antagonists. Science 241:701-3 (1988). Read more (PubMed: 2899909) »
  • Birch PJ  et al. 6,7-Dinitro-quinoxaline-2,3-dion and 6-nitro,7-cyano-quinoxaline-2,3-dion antagonise responses to NMDA in the rat spinal cord via an action at the strychnine-insensitive glycine receptor. Eur J Pharmacol 156:177-80 (1988). Read more (PubMed: 2905271) »
See all 15 Publications for this product

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Answer

The compound may contain larger granules, which will require more effort to dissolve. We would recommend to use heat and sonication.

· Heat in a 50°C water bath for approximately 5 minutes
· Vortex/sonication on removal
· Repeat as necessary

The above method was successfully used to make a 20 mM solution.

Read More

Answer

The molecular weight and formula published on our website represent the molecular weight and formula of the product as depicted in the chemical structure displayed on our website. However, molecular weights (and formulae) can vary slightly from batch to batch - due to water composition, or due to a change of salt. These changes will be indicated on the Certificate of authenticity that accompanies your product. The changes should not affect the biological activity of your product, but it is important that you take them into account when making up solutions. You should therefore always use the batch molecular weight as stated on the Certificate of authenticity or the vial label.

Regarding the solubility issue, gentle warming is recommended that may be able to help the dissolution.

Read More

Answer

Thank you for contacting Abcam and for your interest in our products.


Regarding ab120044 and ab120169, we don't use HPLC to purify the product, it is used only to analyse it.Our methods of purification are proprietary. We would therefore need more clarification about the experiment you are conducting in order to provide further advice.


Please do not hesitate to contact me if you have any additional questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

注册