HRP Anti-CNPase抗体[11-5B] (ab201678)
Key features and details
- HRP Mouse monoclonal [11-5B] to CNPase
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
HRP Anti-CNPase抗体[11-5B]
参阅全部 CNPase 一抗 -
描述
HRP小鼠单克隆抗体[11-5B] to CNPase -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Sheep, Rabbit, Cow, Dog, Pig, Rhesus monkey不与反应: Chicken, Guinea pig -
免疫原
Full length native protein (purified) corresponding to Human CNPase.
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阳性对照
- WB: Human, Mouse and Rat Spinal Cord and Brain tissue lysates. IHC-P: FFPE human normal cerebral cortex tissue sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Batches contain 0.4M arginine. -
Concentration information loading...
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纯度
Affinity purified -
克隆
单克隆 -
克隆编号
11-5B -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab201678于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family. -
细胞定位
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 280752 Cow
- Entrez Gene: 607694 Dog
- Entrez Gene: 1267 Human
- Entrez Gene: 12799 Mouse
- Entrez Gene: 493772 Pig
- Entrez Gene: 25275 Rat
- Entrez Gene: 641337 Rhesus monkey
- Omim: 123830 Human
see all -
别名
- 2'' antibody
- 2'3' cyclic nucleotide 3' phosphodiesterase antibody
- 3''-cyclic-nucleotide 3''-phosphodiesterase antibody
see all
图片
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All lanes : HRP Anti-CNPase antibody [11-5B] (ab201678) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CNPase knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 20 minutesab201678 was shown to specifically react with CNPase in wild-type HAP1 cells as signal was lost in CNPase knockout cells. Wild-type and CNPase knockout samples were subjected to SDS-PAGE. Ab201678 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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IHC image of CNPase staining in a section of formalin-fixed paraffin-embedded human normal cerebral cortex*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab201678 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : HRP Anti-CNPase antibody [11-5B] (ab201678) at 1/5000 dilution
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : Brain (Rat) Tissue Lysate
Lane 6 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 6 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201678 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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