Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling CLN6 with ab82786 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as teh secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - CLN6. Right - Merge.
Western blot - Anti-CLN6 antibody (ab82786)
Anti-CLN6 antibody (ab82786) at 1 µg/ml + 293T cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa Observed band size: 36 kDa Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.