概述

  • 产品名称
  • 描述
    兔多克隆抗体to CLK4
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 107-156 (RSRRSSPKRK RNRHCSSHQS RSKSHRRKRS RSIEDDEEGH LICQSGDVLR) of Human CLK4 (NP_065717).

  • 阳性对照
    • Human fetal brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab104321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
  • 组织特异性
    Expressed in liver, kidney, heart, muscle, brain and endothelial cells.
  • 序列相似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylates on all three types of residues.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI987988 antibody
    • C85119 antibody
    • CDC like kinase 4 antibody
    • CDC-like kinase 4 antibody
    • CLK4 antibody
    • CLK4_HUMAN antibody
    • DKFZp686A20267 antibody
    • Dual specificity protein kinase CLK4 antibody
    • OTTMUSP00000005756; antibody
    • OTTMUSP00000005757 antibody
    • OTTMUSP00000041113 antibody
    • Protein serine threonine kinase Clk4 antibody
    • RP23-9N7.1 antibody
    see all

图片

  • Anti-CLK4 antibody (ab104321) at 1 µg/ml + Human fetal brain lysate at 10 µg

    Predicted band size: 57 kDa



    Gel concentration: 12%
  • Sample type: Human microvascular endothelial cells (25µg) Primary Dilution (ab104321): 1:1000 Secondary Antibody: Goat anti-Rabbit-HRP Secondary Dilution: 1:5000

实验方案

文献

This product has been referenced in:
See all 2 Publications for this product

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colon carcinoma)
Permeabilization
No
Specification
Colon carcinoma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative
Methanol

Dr. Eleni Petsalaki

Verified customer

提交于 Jul 16 2015

Answer

The CLK homology information I received from our sources of the two CLK1 antibodies and the one CLK4 antibody that will be useful for western blotting of human samples is as follows. The percentages refer to percentage identity of amino acids of the antibody immunogens and the three other CLK proteins. Ab104321 anti-CLK4 CLK1: 57% CLK2: 43% CLK3: 43% CLK4: 100% ab71408 anti-CLK1 The immunogen for the CLK1 antibody showed 100% homology with CLK4 but no significant homology for CLK2 or CLK3. ab74044 anti-CLK1 The immunogen homology is 54% with human CLK2, 45% with human CLK3, 77% with CLK4. So ab74044 is likely to have more specificity for CLK1 than will ab71408, which will probably cross-react with CLK4. However, the 77% identity of the ab74044 immunogen with CLK4 is still high. Given that CLK1 and CLK4 are the same molecular weight, it will be impossible to distinguish them from one another in blots. I hope this helps at least a little. If you have any questions, please contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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