重组Anti-CLIP170抗体[EPR5093] (ab134907)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5093] to CLIP170
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CLIP170抗体[EPR5093]
参阅全部 CLIP170 一抗 -
描述
兔单克隆抗体[EPR5093] to CLIP170 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CLIP170 aa 1350-1450 (C terminal). The exact sequence is proprietary.
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阳性对照
- 293T, HeLa, Human fetal kidney, HepG2, A549, HUVEC, and MCF7 cell lysates; Human Hodgkin's lymphoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5093 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134907于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 162 kDa.
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说明 |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 162 kDa. |
靶标
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功能
Seems to be a intermediate filament associated protein that links endocytic vesicles to microtubules. -
组织特异性
Highly expressed in the Reed-Sternberg cells of Hodgkin disease. -
序列相似性
Contains 2 CAP-Gly domains. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton. Localizes to the tips of microtubules. - Information by UniProt
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数据库链接
- Entrez Gene: 6249 Human
- Omim: 179838 Human
- SwissProt: P30622 Human
- Unigene: 524809 Human
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别名
- CAP GLY domain containing linker protein 1 antibody
- CAP-Gly domain-containing linker protein 1 antibody
- CLIP 170 antibody
see all
图片
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All lanes : Anti-CLIP170 antibody [EPR5093] (ab134907) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal kidney tissue lysate
Lane 4 : HepG2 cell lysate
Lane 5 : A549 cell lysate
Lane 6 : HUVEC cell lysate
Lane 7 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Predicted band size: 162 kDa -
Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue sections labeling CLIP170 with purified ab134907 at 1/250 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab134907 被引用在 1 文献中.
- Mouron S et al. Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A. Nat Commun 13:7529 (2022). PubMed: 36477027