Anti-Cleaved PARP1抗体(ab4830)
Key features and details
- Rabbit polyclonal to Cleaved PARP1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cleaved PARP1抗体
参阅全部 Cleaved PARP1 一抗 -
描述
兔多克隆抗体to Cleaved PARP1 -
宿主
Rabbit -
特异性
This antibody specifically recognizes the 85 kDa fragment of cleaved PARP1 and can be used as marker for detecting apoptotic cells. Cleavage site specific antibody, unconjugated. The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP1 and will recognize Asp 214 and Gly 215. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human Cleaved PARP1.
(Peptide available asab10779) -
阳性对照
- WB: THP1 Nuclear Enriched, HeLa Nuclear Enriched, KARPAS-299 and Daudi cell lysate. HeLa and Jurkat cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a peptide spanning the cleavage site to remove antibody that is reactive with full length PARP1. The final product is generated by affinity chromatography using a peptide corresponding to the PARP1 cleavage site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
靶标
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功能
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. -
序列相似性
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
翻译后修饰
Phosphorylated by PRKDC and TXK.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
细胞定位
Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P09874 Human
- Unigene: 177766 Human
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别名
- ADP ribosyltransferase diphtheria toxin like 1 antibody
- ADP ribosyltransferase NAD(+) antibody
- ADP-ribosyltransferase diphtheria toxin-like 1 antibody
see all
图片
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Western blot - Anti-Cleaved PARP1 antibody (ab4830)All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cells treated with Etoposide (1 µM for 16 hours)
Lanes 3 & 5 : HeLa cell lysate
Lane 4 : HeLa cells treated with Etoposide (1 µM for 16 hours)
Lane 6 : HeLa cells treated with Staurosporine (3 µM for 16 hours)
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/14000 dilution
Predicted band size: 85 kDa -
Western blot - Anti-Cleaved PARP1 antibody (ab4830)All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/2000 dilution
Lane 1 : THP1 Nuclear Enriched
Lane 2 : HeLa Nuclear Enriched
Lane 3 : KARPAS-299
Lane 4 : Daudi cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Predicted band size: 85 kDa -
Western blot - Anti-Cleaved PARP1 antibody (ab4830)This image is courtesy of an Abreview submitted by Adam Szadkowski on 26 January 2006.All lanes : Anti-Cleaved PARP1 antibody (ab4830) at 1/1000 dilution
Lane 1 : Non-induced Jurkat cells
Lane 2 : Induced Jurkat cells
Secondary
All lanes : Goat Anti-Rabbit HRP
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 5 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (41)
ab4830 被引用在 41 文献中.
- Tu CC et al. STIM2 knockdown protects against ischemia/reperfusion injury through reducing mitochondrial calcium overload and preserving mitochondrial function. Life Sci 247:116560 (2020). PubMed: 31200000
- Mamriev D et al. A small-molecule ARTS mimetic promotes apoptosis through degradation of both XIAP and Bcl-2. Cell Death Dis 11:483 (2020). PubMed: 32587235
- Sun C et al. FGL1 regulates acquired resistance to Gefitinib by inhibiting apoptosis in non-small cell lung cancer. Respir Res 21:210 (2020). PubMed: 32778129
- Liu L et al. PTEN inhibits non-small cell lung cancer cell growth by promoting G0/G1 arrest and cell apoptosis. Oncol Lett 17:1333-1340 (2019). PubMed: 30655903
- Luo GF et al. FOXD3 may be a new cellular target biomarker as a hypermethylation gene in human ovarian cancer. Cancer Cell Int 19:44 (2019). PubMed: 30858761