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Signal Transduction Protein Trafficking Vesicle Transport Coat Proteins

Anti-Clathrin heavy chain抗体[X22] (ab2731)

  • Datasheet
  • SDS
Reviews (18)Q&A (8)References (65)

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Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)
  • Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)

Key features and details

  • Mouse monoclonal [X22] to Clathrin heavy chain
  • Suitable for: WB, IHC-P, ICC
  • Reacts with: Human, Xenopus laevis
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Clathrin heavy chain抗体[X22]
    参阅全部 Clathrin heavy chain 一抗
  • 描述

    小鼠单克隆抗体[X22] to Clathrin heavy chain
  • 宿主

    Mouse
  • 特异性

    Detects clathrin heavy chain.
  • 经测试应用

    适用于: WB, IHC-P, ICCmore details
  • 种属反应性

    与反应: Human, Xenopus laevis
  • 免疫原

    Full length native protein (purified) corresponding to Human Clathrin heavy chain. Purified human brain clathrin heavy chain.

  • 表位

    Electron microscopy and proteolysis mapping demonstrate that binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    X22
  • 同种型

    IgG1
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Clathrin heavy chain protein (ab112271)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2731于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (5)
1/100 - 1/500. Detects a band of approximately 180 kDa.
IHC-P
1/100.
ICC (2)
1/1000.
说明
WB
1/100 - 1/500. Detects a band of approximately 180 kDa.
IHC-P
1/100.
ICC
1/1000.

靶标

  • 功能

    Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
  • 序列相似性

    Belongs to the clathrin heavy chain family.
  • 细胞定位

    Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession Q00610 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1213 Human
    • Omim: 118955 Human
    • SwissProt: P53675 Human
    • SwissProt: Q00610 Human
    • Unigene: 491351 Human
    • 别名

      • CHC17 antibody
      • Clathrin heavy chain antibody
      • Clathrin heavy chain 1 antibody
      • Clathrin heavy chain 2 antibody
      • Clathrin heavy chain like 1 antibody
      • Clathrin heavy chain on chromosome 17 antibody
      • Clathrin heavy polypeptide antibody
      • CLH 17 antibody
      • CLH 22 antibody
      • CLH-17 antibody
      • CLH1_HUMAN antibody
      • CLH17 antibody
      • CLH22 antibody
      • CLTC antibody
      • CLTCL antibody
      • CLTCL1 antibody
      • CLTCL2 antibody
      • CLTD antibody
      • Hc antibody
      • KIAA0034 antibody
      see all

    图片

    • Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)

      Immunocytochemistry/Immunofluorescence analysis of Clathrin heavy chain shows staining in HeLa cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2731 (1:200) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

    • Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Anti-Clathrin heavy chain antibody [X22] (ab2731) at 1/300 dilution + Human brain lysates at 25 µg

      Secondary
      HRP-conjugated goat anti-mouse IgG + IgM (H+L)

      Developed using the ECL technique.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Immunohistochemistry was performed on normal biopsies of deparaffinized Human colon tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Clathrin Heavy chain ab2731 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)

      Immunocytochemistry/Immunofluorescence analysis of Clathrin heavy chain shows staining in NCI-H460 cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2731 (1:200) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Clathrin Heavy chain ab2731 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)

      Immunocytochemistry/Immunofluorescence analysis of Clathrin heavy chain shows staining in U251 cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2731 (1:200) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

    • Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Immunocytochemistry - Anti-Clathrin heavy chain antibody [X22] (ab2731)

      Xenopus laevis cytoplasmic egg extract visualized live with primary and secondary antibody addition [red is anti-Clathrin heavy chain X22 (ab2731) with goat anti-mouse Alexa Fluor 568 secondary, green is anti-HIP1R (ab77297) with goat anti-rabbit Alexa Fluor 488 secondary]. Large red structures are probably aggregates, but the small structures appear to be specific for vesicle staining.

      See Abreview

    • Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      Western blot - Anti-Clathrin heavy chain antibody [X22] (ab2731)
      All lanes : Anti-Clathrin heavy chain antibody [X22] (ab2731) at 1/500 dilution

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Observed band size: 180 kDa why is the actual band size different from the predicted?
      Additional bands at: 240 kDa, 450 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (65)

    发表研究结果有使用 ab2731?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2731 被引用在 65 文献中.

    • Ren F  et al. NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF-?B signaling pathways. Oncol Lett 19:813-823 (2020). PubMed: 31885716
    • Taverner A  et al. Cholix protein domain I functions as a carrier element for efficient apical to basal epithelial transcytosis. Tissue Barriers 8:1710429 (2020). PubMed: 31928299
    • Moshkanbaryans L  et al. The interaction of assembly protein AP180 and clathrin is inhibited by multi-site phospho-mimetics. Neurochem Int 129:104474 (2019). PubMed: 31129113
    • Cohen EAK  et al. Resolution limit of image analysis algorithms. Nat Commun 10:793 (2019). PubMed: 30770826
    • Berndt-Paetz M  et al. IC/BPS-associated alterations of M2 and M3 muscarinic acetylcholine receptor trafficking in human detrusor. Neurourol Urodyn 38:1818-1827 (2019). PubMed: 31301091
    View all Publications for this product

    客户评价及客户问答

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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-Clathrin antibody [X22] - Membrane Vesicle Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - other (CV1 cells)
    Loading amount
    10 µg
    Specification
    CV1 cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Mr. Nir Drayman

    Verified customer

    提交于 Sep 11 2012

    Western blot abreview for Anti-Clathrin antibody [X22] - Membrane Vesicle Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
    Loading amount
    40 µg
    Specification
    Xenopus laevis cytoplasmic egg extract
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 17 2012

    Western blot abreview for Anti-Clathrin antibody [X22] - Membrane Vesicle Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Platelets)
    Loading amount
    20 µg
    Specification
    Platelets
    Treatment
    ADP for 30 min
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Mahesh Shivananjappa

    Verified customer

    提交于 Nov 24 2011

    Western blot abreview for Anti-Clathrin antibody [X22] - Membrane Vesicle Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (MDCK 2 cells)
    Loading amount
    25 µg
    Specification
    MDCK 2 cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    提交于 Oct 19 2010

    Western blot abreview for Anti-Clathrin antibody [X22] - Membrane Vesicle Marker

    Below Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 02 2006

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