Anti-Claspin抗体(ab3721)

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Western blot - Anti-Claspin antibody (ab3721)

    Key features and details

    • Rabbit polyclonal to Claspin
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Claspin抗体
      参阅全部 Claspin 一抗

    • 描述

      兔多克隆抗体to Claspin

    • 宿主

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • 免疫原

      Synthetic peptide (Human) - which represents a portion of human Claspin encoded within exon 15.

    • 阳性对照

      • HeLa cell lysate.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    性能

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3721 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    WB
    Human
    All applications
    Chimpanzee
    Gorilla
    应用 Ab评论 说明
    WB
    1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
    说明
    WB
    1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).

    靶标

    • 功能

      Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.

    • 序列相似性

      Belongs to the claspin family.

    • 结构域

      The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.

    • 翻译后修饰

      Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Claspin antibody
        • claspin homolog (Xenopus laevis) antibody
        • Claspin homolog antibody
        • CLSPN antibody
        • CLSPN protein antibody
        • CLSPN_HUMAN antibody
        • hClaspin antibody
        • Hu Claspin antibody
        see all

      图片

      • Western blot - Anti-Claspin antibody (ab3721)
        Western blot - Anti-Claspin antibody (ab3721)

        Sample: Hela cell lysate (20µg).

        Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.

        Detection: ECL.

        Sample: Hela cell lysate (20µg).

        Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.

        Detection: ECL.

      文献 (1)

      发表研究结果有使用 ab3721？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab3721 被引用在 1 文献中.

      • Jiang K  et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003). PubMed: 12676962

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