Anti-Claspin抗体(ab3721)

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Immunoprecipitation - Anti-Claspin antibody (ab3721)
  • Western blot - Anti-Claspin antibody (ab3721)

Key features and details

  • Rabbit polyclonal to Claspin
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Claspin抗体
    参阅全部 Claspin 一抗

  • 描述

    兔多克隆抗体to Claspin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Chimpanzee, Gorilla

  • 免疫原

    Synthetic peptide (Human) - which represents a portion of human Claspin encoded within exon 15.

  • 阳性对照

    • HeLa cell lysate.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

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Abpromise™承诺保证使用ab3721于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
IP
Use at 2-10 µg/mg of lysate.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa).
IP
Use at 2-10 µg/mg of lysate.

靶标

  • 功能

    Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.

  • 序列相似性

    Belongs to the claspin family.

  • 结构域

    The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.

  • 翻译后修饰

    Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Claspin antibody
      • claspin homolog (Xenopus laevis) antibody
      • Claspin homolog antibody
      • CLSPN antibody
      • CLSPN protein antibody
      • CLSPN_HUMAN antibody
      • hClaspin antibody
      • Hu Claspin antibody
      see all

    图片

    • Immunoprecipitation - Anti-Claspin antibody (ab3721)
      Immunoprecipitation - Anti-Claspin antibody (ab3721)

      Claspin was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3721 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab3721 at 0.1µg/mL.

      Lane 1: ab3721 IP in HeLa whole cell lysate.

      Lane 2: ab3721 IP in HeLa whole cell lysate.

      Lane 3: Control IgG in HeLa whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 10 seconds

    • Western blot - Anti-Claspin antibody (ab3721)
      Western blot - Anti-Claspin antibody (ab3721)
      All lanes : Anti-Claspin antibody (ab3721) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK293T whole cell lysate at 50 µg

      Predicted band size: 150 kDa


      Exposure time: 30 seconds

    文献 (1)

    发表研究结果有使用 ab3721？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab3721 被引用在 1 文献中.

    • Jiang K  et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003). PubMed: 12676962

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