Anti-Claspin抗体(ab3721)
Key features and details
- Rabbit polyclonal to Claspin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Claspin抗体
参阅全部 Claspin 一抗 -
描述
兔多克隆抗体to Claspin -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla
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免疫原
Synthetic peptide (Human) - which represents a portion of human Claspin encoded within exon 15.
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阳性对照
- HeLa cell lysate.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa). | |
| IP | Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks. -
序列相似性
Belongs to the claspin family. -
结构域
The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1. -
翻译后修饰
Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 63967 Human
- Omim: 605434 Human
- SwissProt: Q9HAW4 Human
- Unigene: 175613 Human
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别名
- Claspin antibody
- claspin homolog (Xenopus laevis) antibody
- Claspin homolog antibody
see all
图片
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Western blot - Anti-Claspin antibody (ab3721)Sample: Hela cell lysate (20
µ g).Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.
Detection: ECL.
Sample: Hela cell lysate (20
µg). Antibody: Affinity purified Rabbit anti-Claspin (ab3721) used at 1:1000 or 1:5000 dilution with (+) or without (-) blocking peptide.
Detection: ECL.
文献 (1)
ab3721 被引用在 1 文献中.
- Jiang K et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003). PubMed: 12676962
