重组Anti-Citrate synthetase抗体[EPR8067] (ab129095)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8067] to Citrate synthetase
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Citrate synthetase抗体[EPR8067]
参阅全部 Citrate synthetase 一抗 -
描述
兔单克隆抗体[EPR8067] to Citrate synthetase -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Citrate synthetase aa 400-500. The exact sequence is proprietary.
Database link: O75390 -
阳性对照
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8067 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129095于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
|
说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
靶标
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通路
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
序列相似性
Belongs to the citrate synthase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 1431 Human
- Entrez Gene: 12974 Mouse
- Entrez Gene: 170587 Rat
- Omim: 118950 Human
- SwissProt: O75390 Human
- SwissProt: Q9CZU6 Mouse
- SwissProt: Q8VHF5 Rat
- Unigene: 430606 Human
see all -
别名
- CISY_HUMAN antibody
- Citrate synthase antibody
- Citrate synthase, mitochondrial antibody
see all
图片
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All lanes : Anti-Citrate synthetase antibody [EPR8067] (ab129095) at 1/10000 dilution (purified)
Lane 1 : HepG2 whole cell lysate
Lane 2 : Caco-2 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling Citrate synthetase with purified ab129095 at a dilution of 1/250. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Citrate synthetase with purified ab129095 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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All lanes : Anti-Citrate synthetase antibody [EPR8067] (ab129095) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue labelling Citrate synthetase with purified ab129095 at a dilution of 1/250. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling Citrate synthetase with purified ab129095 at a dilution of 1/250. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Citrate synthetase with purified ab129095 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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All lanes : Anti-Citrate synthetase antibody [EPR8067] (ab129095) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysates
Lane 2 : Caco-2 cell lysates
Lane 3 : 293T cell lysates
Lane 4 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Citrate synthetase with unpurified ab129095 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Citrate synthetase with unpurified ab129095 at a dilution of 1/250.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (40)
ab129095 被引用在 40 文献中.
- Huynh VN et al. Acute inhibition of OGA sex-dependently alters the networks associated with bioenergetics, autophagy, and neurodegeneration. Mol Brain 15:22 (2022). PubMed: 35248135
- Lovell AJ et al. The effects of diet and chronic exercise on skeletal muscle ghrelin response. Metabol Open 14:100182 (2022). PubMed: 35340718
- Funda J et al. Adipose tissue-specific ablation of PGC-1β impairs thermogenesis in brown fat. Dis Model Mech 15:N/A (2022). PubMed: 35466996
- Bel JS et al. Chronic glucocorticoid exposure causes brown adipose tissue whitening, alters whole-body glucose metabolism and increases tissue uncoupling protein-1. Physiol Rep 10:e15292 (2022). PubMed: 35510321
- Thomas GE et al. The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness. Nat Cell Biol 24:872-884 (2022). PubMed: 35668135