Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% 37.5:1)
Sample
Pig Tissue lysate - other (Skeletal muscle mitochondria purified)
Specification
Skeletal muscle mitochondria purified
Blocking step
3% Casein in PBS with 0.1% Tween-20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X PBS + 0.1% Tween-20
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 926-32213
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/25000
Detection
Detection method
Fluorescence
Bands
Specific: 45 kDa Non-specific: 30, 28 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 27 2014