Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
20 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2%BSA in TBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/100000
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 50 kDa
Additional data
Additional Notes
Lane1: Myoblast, Lane 2: Day 1 of Differentiation, Lane 3: Day 2 of Differentiation
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 26 2011