Anti-Citrate synthetase抗体(ab96600)
Key features and details
- Rabbit polyclonal to Citrate synthetase
- Suitable for: IHC-P, ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Citrate synthetase抗体
参阅全部 Citrate synthetase 一抗 -
描述
兔多克隆抗体to Citrate synthetase -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, IP, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Zebrafish
预测可用于: Cow, Pig, Xenopus laevis
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免疫原
Recombinant fragment within Human Citrate synthetase aa 44-316. The exact sequence is proprietary. NP_938083
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阳性对照
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900) and zebrafish skeletal muscle tissue and mouse liver mitochondrial homogenates. ICC/IF: HeLa and U-2 OS cells. IHC-P: Human colon, mouse intestine, mouse muscle and NCI-N87 xenograft tissues. IP: HEK-293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96600于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
1/100 - 1/1000.
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| IP |
1/100 - 1/500.
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| WB | (8) |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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| 说明 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
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WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
靶标
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通路
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
序列相似性
Belongs to the citrate synthase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 280682 Cow
- Entrez Gene: 1431 Human
- Entrez Gene: 12974 Mouse
- Entrez Gene: 397519 Pig
- Entrez Gene: 170587 Rat
- Entrez Gene: 379886 Xenopus laevis
- Entrez Gene: 322339 Zebrafish
- Omim: 118950 Human
see all -
别名
- CISY_HUMAN antibody
- Citrate synthase antibody
- Citrate synthase, mitochondrial antibody
see all
图片
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Western blot - Anti-Citrate synthetase antibody (ab96600)All lanes : Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa10% SDS-PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody (ab96600)Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Citrate synthetase (green) with ab96600 at a dilution of 1/200.
Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Alpha Tubulin (red) was stained with ab184613 at a dilution of 1/1000. Blue: Hoechst 33342 staining.
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Western blot - Anti-Citrate synthetase antibody (ab96600)Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution + Rat muscle tissue extracts at 50 µg
Predicted band size: 52 kDa10% SDS-PAGE.
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Western blot - Anti-Citrate synthetase antibody (ab96600)Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution + Rat brain tissue extracts at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa10% SDS-PAGE.
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Western blot - Anti-Citrate synthetase antibody (ab96600)Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution + Mouse brain tissue extracts at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa10% SDS-PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody (ab96600)Immunocytochemistry/Immunofluorescence analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labelling Citrate synthetase (green) with ab96600 at a dilution of 1/500.
Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Alpha Tubulin (red) was stained with ab184613 at a dilution of 1/1000. Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (ab96600)IHC image of ab96600 staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96600, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunoprecipitation - Anti-Citrate synthetase antibody (ab96600)Immunoprecipitation of Citrate synthetase from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate using 5 μg of ab96600.
Western blot analysis was performed using ab96600.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (ab96600)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.
Antigen Retrieval: EDTA based buffer, 15 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (ab96600)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse muscle tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (ab96600)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of NCI-N87 xenograft tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.
Antigen Retrieval: EDTA based buffer, 15 mins.
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Western blot - Anti-Citrate synthetase antibody (ab96600)All lanes : Anti-Citrate synthetase antibody (ab96600) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish liver homogenate (20ug)
Lane 3 : Zebrafish skeletal muscle homogenate (20ug)
Lane 4 : Mouse liver mitochondrial homogenate (20ug)
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (117)
ab96600 被引用在 117 文献中.
- Mendham AE et al. Exercise training improves mitochondrial respiration and is associated with an altered intramuscular phospholipid signature in women with obesity. Diabetologia N/A:N/A (2021). PubMed: 33770195
- Laquatra C et al. HIF1a-dependent induction of the mitochondrial chaperone TRAP1 regulates bioenergetic adaptations to hypoxia. Cell Death Dis 12:434 (2021). PubMed: 33934112
- D'Angelo L et al. NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate. Cell Rep 35:109002 (2021). PubMed: 33882309
- Hahn D et al. Nox4 Knockout Does Not Prevent Diaphragm Atrophy, Contractile Dysfunction, or Mitochondrial Maladaptation in the Early Phase Post-Myocardial Infarction in Mice. Cell Physiol Biochem 55:489-504 (2021). PubMed: 34416105
- Broniarek I et al. The Influence of Statins on the Aerobic Metabolism of Endothelial Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32098258