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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH

Anti-CIKS/ACT1抗体(ab5974)

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Western blot - Anti-CIKS/ACT1 antibody (ab5974)
  • Immunocytochemistry/ Immunofluorescence - Anti-CIKS/ACT1 antibody (ab5974)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIKS/ACT1 antibody (ab5974)

Key features and details

  • Rabbit polyclonal to CIKS/ACT1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-CIKS/ACT1抗体
    参阅全部 CIKS/ACT1 一抗
  • 描述

    兔多克隆抗体to CIKS/ACT1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide:

    PPQLQETRMNRSIP

    , corresponding to amino acids 2-15 of Human Act1.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • Human lung and placental tissue lysates.
  • 常规说明

    This product was previously labelled as CIKS

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CIKS/ACT1 protein (ab161141)

应用

Our Abpromise guarantee covers the use of ab5974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 65 kDa).

靶标

  • 功能

    Could be involved in the activation of both NF-kappa-B via a NF-kappa-B inhibitor kinase (IKK)-dependent mechanism and stress-activated protein kinase (SAPK)/JNK.
  • 组织特异性

    Widely expressed.
  • 序列相似性

    Contains 1 SEFIR domain.
  • Target information above from: UniProt accession O43734 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10758 Human
    • Omim: 607043 Human
    • SwissProt: O43734 Human
    • Unigene: 561514 Human
    • 别名

      • Act 1 antibody
      • ACT1 antibody
      • Activator of cAMP responsive element modulator (CREM) in testis antibody
      • Adapter protein CIKS antibody
      • C6ORF4 antibody
      • C6ORF5 antibody
      • C6ORF6 antibody
      • Chromosome 6 Open Reading Frame 4 antibody
      • Chromosome 6 Open Reading Frame 5 antibody
      • Chromosome 6 Open Reading Frame 6 antibody
      • CIKS_HUMAN antibody
      • Connection to IKK and SAPK / JNK antibody
      • Connection to IKK and SAPK/JNK antibody
      • Nuclear Factor Kappa B Activator 1 antibody
      • Nuclear factor NF kappa B activator 1 antibody
      • Nuclear factor NF-kappa-B activator 1 antibody
      • TRAF3 interacting protein 2 antibody
      • TRAF3-interacting protein 2 antibody
      • TRAF31P2 antibody
      • TRAF3IP2 antibody
      see all

    图片

    • Western blot - Anti-CIKS/ACT1 antibody (ab5974)
      Western blot - Anti-CIKS/ACT1 antibody (ab5974)

      Western blot analysis of CIKS/ACT1 expression in human lung (lane A) and placenta (lane B) tissue lysates withab5974 at 1 µg /ml. Western blot analysis of CIKS/ACT1 expression in human lung (lane A) and placenta (lane B) tissue lysates withab5974 at 1 µg /ml.

    • Immunocytochemistry/ Immunofluorescence - Anti-CIKS/ACT1 antibody (ab5974)
      Immunocytochemistry/ Immunofluorescence - Anti-CIKS/ACT1 antibody (ab5974)

      Immunofluorescence of CIKS/ACT1 in Human lung tissue with ab5974 at 20 ug/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIKS/ACT1 antibody (ab5974)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIKS/ACT1 antibody (ab5974)

      ab5974 at 5µg/ml staining CIKS/ACT1 in human lung tissue by  IHC

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (0)

    发表研究结果有使用 ab5974?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5974 尚未被引用在任何文献中。

    客户评价及客户问答

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    There are currently no Customer reviews or Questions for ab5974.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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