为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Cell Biology Apoptosis Intracellular Survivin / IAPs

Anti-cIAP1抗体(ab2399)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (14)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-cIAP1 antibody (ab2399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)

Key features and details

  • Rabbit polyclonal to cIAP1
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

你可能会感兴趣

蛋白
Product image
Recombinant Human cIAP1 protein (ab114410)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-cIAP1抗体
    参阅全部 cIAP1 一抗
  • 描述

    兔多克隆抗体to cIAP1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. (Peptide available as ab38272.)

  • 阳性对照

    • WB: Jurkat cell lysate, PC12 cell lysate, rat kidney tissue lysate
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.03% Proclin 300
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cell Death
    • Necroptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human cIAP1 protein (ab114410)

应用

Our Abpromise guarantee covers the use of ab2399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).

靶标

  • 功能

    Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
  • 组织特异性

    Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
  • 序列相似性

    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 CARD domain.
    Contains 1 RING-type zinc finger.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession Q13490 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 329 Human
    • Entrez Gene: 11797 Mouse
    • Entrez Gene: 60371 Rat
    • Omim: 601712 Human
    • SwissProt: Q13490 Human
    • SwissProt: Q62210 Mouse
    • Unigene: 696238 Human
    • Unigene: 335659 Mouse
    • Unigene: 205955 Rat
    see all
  • 别名

    • API 1 antibody
    • API1 antibody
    • Apoptosis inhibitor 1 antibody
    • Baculoviral IAP repeat containing 2 antibody
    • Baculoviral IAP repeat containing protein 2 antibody
    • Baculoviral IAP repeat-containing protein 2 antibody
    • BIRC 2 antibody
    • BIRC2 antibody
    • BIRC2_HUMAN antibody
    • C IAP1 antibody
    • C-IAP1 antibody
    • Cellular inhibitor of apoptosis 1 antibody
    • cellular inhibitor of apoptosis protein 1 antibody
    • cIAP 1 antibody
    • cIAP1 antibody
    • HIAP 2 antibody
    • HIAP-2 antibody
    • HIAP2 antibody
    • IAP 2 antibody
    • IAP homolog B antibody
    • IAP-2 antibody
    • IAP2 antibody
    • Inhibitor of apoptosis protein 2 antibody
    • MIHB antibody
    • NFR2 TRAF signalling complex protein antibody
    • RING finger protein 48 antibody
    • RNF 48 antibody
    • RNF48 antibody
    • TNFR2 TRAF signaling complex protein 2 antibody
    • TNFR2-TRAF-signaling complex protein 2 antibody
    see all

图片

  • Western blot - Anti-cIAP1 antibody (ab2399)
    Western blot - Anti-cIAP1 antibody (ab2399)
    Anti-cIAP1 antibody (ab2399) + Jurkat Cell lysate

    Predicted band size: 69 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)
    IHC image of ab2399 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (14)

    发表研究结果有使用 ab2399?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2399 被引用在 14 文献中.

    • Craver BM  et al. The SMAC mimetic LCL-161 selectively targets JAK2V617F mutant cells. Exp Hematol Oncol 9:1 (2020). PubMed: 31908904
    • Leung DTH  et al. Combined PPAR? Activation and XIAP Inhibition as a Potential Therapeutic Strategy for Ovarian Granulosa Cell Tumors. Mol Cancer Ther 18:364-375 (2019). PubMed: 30530769
    • Cheng Z  et al. Lactobacillus raisesin vitroanticancer effect of geniposide in HSC-3 human oral squamous cell carcinoma cells. Exp Ther Med 14:4586-4594 (2017). PubMed: 29104666
    • Xu Q  et al. The cytoplasmic nuclear receptor RAR? controls RIP1 initiated cell death when cIAP activity is inhibited. Nat Commun 8:425 (2017). PubMed: 28871172
    • Balakrishnan K  et al. Reactivation of Smac-mediated apoptosis in chronic lymphocytic leukemia cells: mechanistic studies of Smac mimetic. Oncotarget 7:39458-39472 (2016). PubMed: 27223062
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Question

    the monoclonal antibody we are using is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu28 of human c-IAP1 protein.

    The polyclonalantibody we are usingis produced by immunizing animals with a synthetic peptide corresponding to a region between the first two BIR domains of c-IAP1.

    Thank you for your help.

    Sincerely yours,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 17 2012

    Answer

    Thank you for your email.

    The peptide is from the sequence around amino acid 530 of human cIAP1. This sequence does not match with the immunogen sequence of polyclonal and monoclonal antibodies you have so this peptide ab38272 would be of no use.

    If you are interested in doing blocking experiment then ab38272 would block ab2399.

    I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Sep 17 2012

    Question

    I would like to test the specificity ofone of our anti-cIAP1 mAb by using your cIAP1 blocking peptide.

    I would therefore know if this peptide can be used as an immunogen for other antibodies than https://www.abcam.com/index.html?datasheet=2399.

    Thank you in advance for your kind reply.

    Sincerely yours,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 14 2012

    Answer

    Thank you for contacting us.

    Could you please explain what do you mean by other antibodies? The antibodies against the same peptide should be same however difference can be seen in specificity, purity etc.

    The peptide ab38272 is from a sequence flanking 530 of human cIAP1. We unfortunately don't know the exact sequence so this peptide may not able to block your antibody.

    Do you know the immunogen sequence of your antibody?

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Sep 14 2012

    Question

    samples: lysates of several human leucemic B-cells (BJAB, I-83, ...) ab2399 and ab32059: sees a band at 35 kDa, but not at 70 kDa as expected Ab: 1/50, 1/1000 overnight 4 degree regular PAGE system and Millipore's snap-system - same results secondary: OK block: 5% milk ab8421: sees high background and band at 55 kDa (however, band is not dominant), but all bands are of equal intensity Ab: 1/1000 and higher regular PAGE system and Millipore's snap-system - same results secondary: OK block: 5% milk

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2011

    Answer

    Thank you for your phone call. I am very sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate these 3 cases and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. As we discussed, I believe these 3 products should have given satisfactory results. It appears that you may have received either faulty vials, or the shipment was affected by temperature variations, customs or other unknown factors. I apologize very much for the inconvenience, but am pleased to let you know that I have arranged for the replacement shipment as we discussed. Thank you for your understanding. I wish you good luck with your research! Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    回复于 Nov 21 2011

    Question

    I know the datasheet lists the immunogen as peptide ab38272, but I was wondering if it were possible to find out the exact sequence of the peptide used as the immunogen. I understand this isn't always possible, but please let me know if it is. Than

    Read More

    Abcam community

    Verified customer

    Asked on Oct 11 2011

    Answer

    Thank you for your inquiry. We source this antibody from an outside laboratory, and unfortunately we do not have access to the exact sequence used for the immunogen. If you plan to use the antibody on a fragment of the protein, we may be able to get more specific information of whether this antibody would bind to your fragment, but unfortunately we don't have access to any further information than what is on the datasheet. I apologize that I cannot be more help

    Read More

    Abcam Scientific Support

    回复于 Oct 11 2011

    Question

    We would like to know which region of cIAP1 does the antibody recognize (i.e. immunogen). Thanks!

    Read More

    Abcam community

    Verified customer

    Asked on Apr 27 2006

    Answer

    Thank you for your enquiry. The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 May 02 2006

    Question

    i want to use it for immunohistochemistry, and have no idea whether it will work or not, does anybody used to try it on IHC?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 07 2004

    Answer

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

    Read More

    Abcam Scientific Support

    回复于 Oct 07 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有