Key features and details
- Rabbit polyclonal to cIAP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
参阅全部 cIAP1 一抗
经测试应用适用于: IHC-P, WBmore details
种属反应性与反应: Mouse, Rat, Human
The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. (Peptide available as ab38272.)
- WB: Jurkat cell lysate, PC12 cell lysate, rat kidney tissue lysate
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol, 0.5% BSA, 0.015% EDTA
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab2399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).|
功能Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
组织特异性Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
序列相似性Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 CARD domain.
Contains 1 RING-type zinc finger.
- Information by UniProt
- API 1 antibody
- API1 antibody
- Apoptosis inhibitor 1 antibody
Anti-cIAP1 antibody (ab2399) + Jurkat Cell lysate
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
IHC image of ab2399 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab2399 被引用在 13 文献中.
- Craver BM et al. The SMAC mimetic LCL-161 selectively targets JAK2V617F mutant cells. Exp Hematol Oncol 9:1 (2020). PubMed: 31908904
- Cheng Z et al. Lactobacillus raisesin vitroanticancer effect of geniposide in HSC-3 human oral squamous cell carcinoma cells. Exp Ther Med 14:4586-4594 (2017). PubMed: 29104666
- Xu Q et al. The cytoplasmic nuclear receptor RAR? controls RIP1 initiated cell death when cIAP activity is inhibited. Nat Commun 8:425 (2017). PubMed: 28871172
- Balakrishnan K et al. Reactivation of Smac-mediated apoptosis in chronic lymphocytic leukemia cells: mechanistic studies of Smac mimetic. Oncotarget 7:39458-39472 (2016). PubMed: 27223062
- Zeng W et al. Silencing of hERG1 Gene Inhibits Proliferation and Invasion, and Induces Apoptosis in Human Osteosarcoma Cells by Targeting the NF-?B Pathway. J Cancer 7:746-57 (2016). WB ; Human . PubMed: 27076857