Key features and details
- Goat polyclonal to CHRNB4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
参阅全部 CHRNB4 一抗
经测试应用适用于: IHC-P, WBmore details
预测可用于: Mouse, Rat, Horse, Hamster, Cow, Dog, Pig, Gorilla, Opossum, Common marmoset, Elephant
- Human uterus tissue.
This product was previously labelled as Nicotinic Acetylcholine Receptor beta 4
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab129276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Preliminary experiments gave an approx 40kD band in human testis lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kD according to NP_000741.1. The 40kD band was successfully blocked by incubation with the immunizing peptide.|
功能After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
序列相似性Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-4/CHRNB4 sub-subfamily.
细胞定位Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- acetylcholine receptor, nicotinic, beta 4 (neuronal) antibody
- ACHB4_HUMAN antibody
- AChR antibody
ab129276 被引用在 5 文献中.
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- Qian J et al. Nicotinic acetylcholine receptors (nAChRs) are expressed in Trpm5 positive taste receptor cells (TRCs). PLoS One 13:e0190465 (2018). PubMed: 29293602
- Qian J et al. Nicotinic acetylcholine receptor (CHRN) expression and function in cultured human adult fungiform (HBO) taste cells. PLoS One 13:e0194089 (2018). PubMed: 29513745
- Qian J et al. Nicotine-Induced Effects on Nicotinic Acetylcholine Receptors (nAChRs), Ca2+ and Brain-Derived Neurotrophic Factor (BDNF) in STC-1 Cells. PLoS One 11:e0166565 (2016). ICC/IF ; Mouse . PubMed: 27846263