Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton in PBS
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blocking buffer
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/100
Additional data
Additional Notes
ABTRIAL-EBRAG
Abcam response
We are sorry to hear that this antibody didn't work well in this application and with these samples. We have followed up with the customer in accordance to our Abpromise (www.abcam.com/abpromise).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 09 2014