ChIP试剂盒(ab500)
概述
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产品名称ChIP试剂盒
参阅全部 ChIP Kit 试剂盒 -
样品类型Adherent cells, Suspension cells
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产品概述
ChIP kit ab500 provides a protocol and reagents for running ChIP assays including:
- cell lysis and chromatin extraction
- chromatin shearing and DNA fragment length analysis
- immunoprecipitation and DNA purification
DNA produced using the kit can be analyzed using qPCR.
The kit has been validated for ChIP assays with mammalian samples.
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说明
This kit uses Protein A sepharose beads for antibody pulldown. See table below for Protein A and Protein G binding affinities with antibodies from commonly used species. For other species of antibody, consult the table in the protocol booklet.
Species raised in
Isotype Protein A binding affinity Protein G binding affinity Rabbit All isotypes +++ ++ Goat All isotypes - ++ Mouse
IgG1 + +++ IgG2a +++ +++ IgG2b ++ ++ IgG3 + + IgM Use anti-mouse IgM ChIP assay products and guides
Find more ChIP assay / chromatin immunoprecipitation resources and products, ChIP antibody products, and other ChIP assay kits and related reagents.
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经测试应用适用于: ChIPmore details
不适用于: CHIPseq
性能
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存放说明Please refer to protocols.
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存储溶液Kit Constituents: Please see protocol.
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组件 1 kit Buffer A 1 x 10ml Buffer B 1 x 30ml 1.25M Glycine 1 x 10ml Buffer C 1 x 30ml Buffer D 1 x 3ml ChIP Buffer 5X 1 x 84ml DNA Purifying Slurry 1 x 3ml ab1791 - H3 antibody 1 x 25µg PCR-Grade Water 1 x 10.2ml Protease Inhibitors 1 tablet Protein A Beads Unblocked 1 x 960µl Proteinase K 1 x 30µl -
研究领域
相关产品
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Related Products
应用
Our Abpromise guarantee covers the use of ab500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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ChIP | Use at an assay dependent concentration. |
图片
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Chromatin immunoprecipitation assay was performed to define the interaction of serum response factor (SRF) with the intragenic serum response element (SRE) regulatory motif in mouse using ab500 ChIP Kit
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Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 (tri methyl K9) antibody (ab8898). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 2 µg of ab8898 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of transcribed region.
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Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 (tri methyl K4) antibody (ab12209). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 5 µg of ab12209 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci). Primers and probes are located in the first kb of the transcribed region.
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Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 antibody (ab1791). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 2 µg of ab1791 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
实验方案
文献
This product has been referenced in:
- Islam SS et al. Eugenol potentiates cisplatin anti-cancer activity through inhibition of ALDH-positive breast cancer stem cells and the NF-?B signaling pathway. Mol Carcinog 57:333-346 (2018). Read more (PubMed: 29073729) »
- Zhou A et al. RNA Binding Protein, HuR, Regulates SCN5A Expression Through Stabilizing MEF2C transcription factor mRNA. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29678826) »