鸡抗兔IgG H&L (TRITC) (ab6826)

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Key features and details

  • Chicken Anti-Rabbit IgG H&L (TRITC)
  • Conjugation: TRITC. Ex: 547nm, Em: 572nm
  • Host species: Chicken
  • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

Conjugates logo Related conjugates and formulations

Biotin FITC HRP Texas Red ® Unconjugated

概述

  • 产品名称

    鸡抗兔IgG H&L (TRITC)
    参阅全部 IgG 二抗

  • 宿主

    Chicken

  • 靶标种属

    Rabbit

  • 经测试应用

    适用于: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

  • 免疫原

    Rabbit IgG, whole molecule

  • 偶联物

    TRITC. Ex: 547nm, Em: 572nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.

  • 共轭说明

    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6826于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
说明
应用说明
suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab6826？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6826 被引用在 1 文献中.

  • Prozialeck WC  et al. Evaluation of cystatin C as an early biomarker of cadmium nephrotoxicity in the rat. Biometals 29:131-46 (2016). PubMed: 26715107

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Question

ORDER NUMBER 114090 DESCRIPTION OF THE PROBLEM weak Non-specific staining SAMPLE Hela PRIMARY ANTIBODY ab2893 DETECTION METHOD fluorescent microscoop POSITIVE AND NEGATIVE CONTROLS USED pos control radiation with UV during 45 min. neg control no primary antibody ANTIBODY STORAGE CONDITIONS refrigerator FIXATION OF SAMPLE methanol 10min at -20?C paraformaldehyde during 30 min at roomtemp PERMEABILIZATION STEP triton-X 100 ice cold for 15 min BLOCKING CONDITIONS 1h 1%BSA in PBS SECONDARY ANTIBODY ab6826 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 11 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? fixation time and method blocking time dilution different dilutions of primary and secondary antibody ADDITIONAL NOTES Purchase order number 4203309615 I have changed different steps in the protocol. Nothing worked!! I always got the same red background. It always coloured the entire cell!! Even in the neg control. as a last resort, I tried another sec antibody. this antibody gave the correct result.(compared with literature) I can say that the secondary antibody does noth work at all.

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Answer

I'm sorry to hear you are experiencing problems with ab6826, the fact that a "no primary" control gave you high background does indeed point towards a non-specificity of a damaged vial. Could you please clarify what dilution of this secondary antibody you have tried and how long you incubated it for? Thank you for providing this piece of information which will help me understand your problem better, I look forward to hearing from you to resolve this matter,

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Question

I know it says purified IgG, but has it been affinity purified so that it does not cross react with other species. The antibody will be mixed with donkey, goat and other secondary antibodies

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Answer

All the information avaliable for this product is stated on the datasheet. The datasheet reads "This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose". Therefore this antibody will be specific for Rabbit Immunoglobulin.

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Question

Hi, there, I am gonna do the immunostaining on the human cell lines, the primary antibody I have now was raised in rabbit, and I need a secondary anti-rabbit antibody conjugated with rhogamine, on your list, there are so many secondary antibodis fit my requirments, but raised in different animals (donkey #6799, goat #6718, sheep #6792, and chicken #6826), could you please tell me which one I should use if I want to have the lowest backgroung (crossreaction) from the secondary antibody on the human cells? Thanks a lot Helen

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Answer

There should be no difference between any of these secondaries; they will all work similarly well. However, if you wish to reduce background with the human cells then we suggest using secondaries that have been pre-adsorbed against human serum, thus, Goat polyclonal to rabbit IgG H&L (rhodamine) Pre-Adsorbed https://www.abcam.com/go.cfm?p=4&Ab=7087 and Donkey polyclonal to rabbit IgG H&L (rhodamine) Pre-Adsorbed https://www.abcam.com/go.cfm?p=4&Ab=7080 These should give you the lowest background.

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