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Immunology Innate Immunity Chemokines Beta Chemokine Rec. (CCR)

Anti-Chemokine Receptor D6抗体(ab1656)

  • Datasheet
  • SDS
Reviews (4)Q&A (5)References (4)

Product price, shipping and contact information

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chemokine Receptor D6 antibody (ab1656)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chemokine Receptor D6 antibody (ab1656)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chemokine Receptor D6 antibody (ab1656)

Key features and details

  • Goat polyclonal to Chemokine Receptor D6
  • Suitable for: IHC-P
  • Reacts with: Mouse
  • Isotype: IgG

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二抗
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

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概述

  • 产品名称

    Anti-Chemokine Receptor D6抗体
    参阅全部 Chemokine Receptor D6 一抗
  • 描述

    山羊多克隆抗体to Chemokine Receptor D6
  • 宿主

    Goat
  • 特异性

    Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region.
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    See all applications and species data
  • 免疫原

    Synthetic peptide:

    SSSIYDYDYLDDMTILVCRKDEVLSFGRC

    , corresponding to amino acids 18-45 of Mouse Chemokine Receptor D6
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • Mouse spleen cells or lung.
  • 常规说明


    Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Mouse
应用 Ab评论 说明
IHC-P
1/250.
说明
IHC-P
1/250.

靶标

  • 功能

    Receptor for C-C type chemokines including SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3.
  • 组织特异性

    Expressed primarily in placenta and fetal liver, and found at very low levels in the lung and lymph node. Found in endothelial cells lining afferent lymphatics in dermis and lymph nodes. Also found in lymph nodes subcapsular and medullary sinuses, tonsillar lymphatic sinuses and lymphatics in mucosa and submucosa of small and large intestine and appendix. Also found in some malignant vascular tumors.
  • 序列相似性

    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession O00590 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 59289 Mouse
    • SwissProt: O08707 Mouse
    • Unigene: 258105 Mouse
    • 别名

      • C C chemokine receptor D6 antibody
      • C-C chemokine receptor D6 antibody
      • CC chemokine binding receptor JAB61 antibody
      • CC chemokine receptor D6 antibody
      • CCBP 2 antibody
      • CCBP2 antibody
      • CCBP2_HUMAN antibody
      • CCR 10 antibody
      • CCR 9 antibody
      • CCR10 antibody
      • CCR9 antibody
      • Chemokine (C C motif) receptor 9 antibody
      • Chemokine (C C) receptor 9 antibody
      • Chemokine binding protein 2 antibody
      • Chemokine binding protein D6 antibody
      • Chemokine C C motif receptor 9 antibody
      • Chemokine C C receptor 9 antibody
      • Chemokine CC motif receptor 9 antibody
      • Chemokine CC receptor 9 antibody
      • Chemokine receptor 9 antibody
      • Chemokine receptor CCR 10 antibody
      • Chemokine receptor CCR 9 antibody
      • Chemokine receptor CCR-10 antibody
      • Chemokine receptor CCR-9 antibody
      • Chemokine receptor CCR10 antibody
      • Chemokine receptor CCR9 antibody
      • Chemokine receptor D6 antibody
      • Chemokine-binding protein 2 antibody
      • Chemokine-binding protein D6 antibody
      • CMKBR 9 antibody
      • CMKBR9 antibody
      • D6 antibody
      • hD6 antibody
      • MGC126678 antibody
      • MGC138250 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chemokine Receptor D6 antibody (ab1656)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chemokine Receptor D6 antibody (ab1656)
      ab1656, at a dilution of 1/250, staining Chemokine Receptor D6 in mouse spleen section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chemokine Receptor D6 antibody (ab1656)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chemokine Receptor D6 antibody (ab1656)
      ab1656, at a dilution of 1/250, staining Chemokine Receptor D6 in mouse lung section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chemokine Receptor D6 antibody (ab1656)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chemokine Receptor D6 antibody (ab1656)
      ab1656, at a dilution of 1/250, staining Chemokine Receptor D6 in mouse spleen section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (4)

    发表研究结果有使用 ab1656?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1656 被引用在 4 文献中.

    • Hajrasouliha AR  et al. Expression of the chemokine decoy receptor D6 mediates dendritic cell function and promotes corneal allograft rejection. Mol Vis 19:2517-25 (2013). Flow Cyt ; Mouse . PubMed: 24357920
    • Besusso D  et al. BDNF-TrkB signaling in striatopallidal neurons controls inhibition of locomotor behavior. Nat Commun 4:2031 (2013). IHC . PubMed: 23774276
    • Wang B  et al. Dlx6 regulates molecular properties of the striatum and central nucleus of the amygdala. J Comp Neurol 519:2320-34 (2011). PubMed: 21452241
    • Kin NW  et al. DNA Microarray Gene Expression Profile of Marginal Zone versus Follicular B Cells and Idiotype Positive Marginal Zone B Cells before and after Immunization with Streptococcus pneumoniae. J Immunol 180:6663-74 (2008). WB, Flow Cyt ; Mouse . PubMed: 18453586

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-9 of 9 Abreviews or Q&A

    Flow Cytometry abreview for Anti-Chemokine Receptor D6 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Cynomolgus Monkey Cell (Whole blood)
    Specification
    Whole blood
    Preparation
    Cell harvesting/tissue preparation method: After red blood cell lysis
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 27 2012

    Question

    ReviewID: xxx AbID: 1656, Anti-Chemokine Receptor D6 antibody Rating: Poor Image: Anti-Chemokine Receptor D6 antibody for Flow Cytometry (Rat) Sample: Species: Rat Type: Cell Specification: Splenocytes Preparation: Cell harvesting/tissue preparation method: spleen was mashed
    Sample buffer: PBS/BSA 1%/Azide 0.01% Application: Fixation: Permeabilization: No Gating Strategy: alive lymphocytes Other detail: Dilution: 1/100 Incubation time: 1 hour at 4°C Diluent: PBS/BSA 1%/Azide 0.01% Secondary Antibody: Name: Non-Abcam Antibody was used: donkey biotin anti goat Conjugation: Biotin Dilution: 1/200 Additional Data: Additional Notes: streptavidin PercP was used as tertiary antibody (1/200)

    Read More

    Abcam community

    Verified customer

    Asked on Apr 20 2012

    Answer

    Thank you for submitting an Abreview of ab1656. As you may have noticed, your review has now been published on our website.
    Since you obtained poor results using the antibody in a untested species, we would like to follow up on this to see if we can possibly improve the results by offering some troubleshotting advice.
    Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:
    - the homology of the immunogen with rat is 89%, thus, theoretically might work, but dependend on where exactly the epitope the binding might be weak.
    - check if the protein is expressed with high enough copy numbers in this sample type
    - incubate longer with the primary antibody
    I thank you for your Abreview submission and wish you good luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Apr 20 2012

    Flow Cytometry abreview for Anti-Chemokine Receptor D6 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rat Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: spleen was mashed
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 19 2012

    Question

    Do you have any IHC-P images where you used a blocking peptide with this antibody to show specific staining?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 09 2012

    Answer

    Thank you for calling Abcam yesterday.
    I have talked to the lab and I am sorry but we do not have an immunohistochemistry image showing the use of the blocking peptide with the antibody.
    As we talked about yesterday as well, please find below the links that explain about our Abpromise and our testing discount program:
    https://www.abcam.com/index.html?pageconfig=abpromise
    https://www.abcam.com/index.htmlpageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
    If there is anything else I can help you with, or if you would like to take advantage of our testing discount program to try ab1656 in western blot, please let me know.

    Read More

    Abcam Scientific Support

    回复于 Feb 09 2012

    Flow Cytometry abreview for Anti-Chemokine Receptor D6 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (cells from blood)
    Specification
    cells from blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Permeabilization
    No
    Gating Strategy
    on lymphocytes
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 20 2011

    Flow Cytometry abreview for Anti-Chemokine Receptor D6 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMC)
    Specification
    PBMC
    Preparation
    Cell harvesting/tissue preparation method: from whole blood
    Sample buffer: PBS/BSA 2% sodium azide 0.01%
    Fixation
    Formaldehyde
    Permeabilization
    No
    Gating Strategy
    lymphocytes
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 08 2010

    Question

    Thank you for your providing the method to do the staining with D6 antibody. I bought the antigen retrieval solution from DAKO and do the staining again accorrding to your protocol. Unfortunately, I can not see the positive results on the wt tissues(lung and spleen) compared to the D6 ko tissues( Nature immunology 2005). So I do not think this antibody worked. Do you have any other suggestions? I have already tried four different times experiments on different kinds of tissues( fresh, paraffin embedded and free floating, antigen retrivel et al). It seemed that it is just a waste of time. So I give up and just want to get refund for this antibody. Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 23 2005

    Answer

    Thank you for your email and I'm sorry to hear that you are still experiencing difficulty with ab1656. You should definitely see positive staining with the spleen and lung tissue and so it seems as if the antibody is damaged in some way. I can have a refund issued to you - can you please give me the purchase order number that was used when you ordered this antibody? Thank you and I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    回复于 Sep 26 2005

    Question

    Customer wrote the following email on Sept 13, but did not receive the response (email address was incorrect): I bought this antibody 1 month ago, and do the staining exactly according to your protocols. I try fresh tissue and paraffin embedded tissues. They all did not work. How about your special protocol for this antibody ? Do you have any positive slides I can use for this antibody? I just want it worked, I do not want the refund. This is why I want to buy this antibody. I will appreciate you if you can help me to figure it out. However, if it does not work, I still want my refund which you promised me before I bought this antibody. Thank you very much.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 21 2005

    Answer

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1656. Our source for this antibody was able to provide me with the IHC-P protocol below, which I hope will be helpful. Also, mouse spleen or lung tissue is recommended as a positive control. If you need additional assistance, please contact us again. Immunohistochemistry on Paraffin sections: Paraffin sections of tissues fixed in 10% formalin were used for testing antibodies. Preparation of slides: Place slides in 60 C oven overnight after cutting. Then follow the procedure below: 1) xylene 2x10min 2) 100% alcohol 2x10min 3) 95% alcohol 5 min 4) 80% alcohol 5 min 5) rinse in distilled water 2x2 min 6) antigen retrieval if necessary 7) Immunohistochemistry Antigen Retrieval: Sections were treated with antigen retrieval solution (Dako # S-1700, pH 6.0 or #S-3308, pH 8.0) and steamed for 20 minutes followed by cooling for 20 minutes. Immunohistochemistry: Block the endogenous peroxide by incubating the sections with 3% H2O2 for 15 minutes at room temperature (RT). Wash the sections with PBS and incubate with 2% normal rabbit serum at room temperature to block the non-specific binding sites. Wash the section with PBS and incubate with primary antibody (anti-chemokine receptor) at recommended dilution for 60 minutes at RT. Wash the slides and incubate at RT with biotinylated rabbit anti-goat IgG H+L (Vector Labs) at 1:200.

    Read More

    Abcam Scientific Support

    回复于 Sep 21 2005

    Question

    I bought this antibody 1 month ago, and do the staining exactly according to your protocols. I try fresh tissue and paraffin embedded tissues. They all did not work. How about your special protocol for this antibody ? Do you have any positive slides I can use for this antibody? I just want it worked, I do not want the refund. This is why I want to buy this antibody. I will appreciate you if you can help me to figure it out. However, if it does not work, I still want my refund which you promised me before I bought this antibody. Thank you very much.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 13 2005

    Answer

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1656. Our source for this antibody was able to provide me with the IHC-P protocol below, which I hope will be helpful. Also, mouse spleen or lung tissue is recommended as a positive control. If you need additional assistance, please contact us again. Immunohistochemistry on Paraffin sections: Paraffin sections of tissues fixed in 10% formalin were used for testing antibodies. Preparation of slides: Place slides in 60 C oven overnight after cutting. Then follow the procedure below: 1) xylene 2x10min 2) 100% alcohol 2x10min 3) 95% alcohol 5 min 4) 80% alcohol 5 min 5) rinse in distilled water 2x2 min 6) antigen retrieval if necessary 7) Immunohistochemistry Antigen Retrieval: Sections were treated with antigen retrieval solution (Dako # S-1700, pH 6.0 or #S-3308, pH 8.0) and steamed for 20 minutes followed by cooling for 20 minutes. Immunohistochemistry: Block the endogenous peroxide by incubating the sections with 3% H2O2 for 15 minutes at room temperature (RT). Wash the sections with PBS and incubate with 2% normal rabbit serum at room temperature to block the non-specific binding sites. Wash the section with PBS and incubate with primary antibody (anti-chemokine receptor) at recommended dilution for 60 minutes at RT. Wash the slides and incubate at RT with biotinylated rabbit anti-goat IgG H+L (Vector Labs) at 1:200.

    Read More

    Abcam Scientific Support

    回复于 Sep 14 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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