重组Anti-CHD5抗体[EPR23858-3] (ab259857)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23858-3] to CHD5
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CHD5抗体[EPR23858-3]
参阅全部 CHD5 一抗 -
描述
兔单克隆抗体[EPR23858-3] to CHD5 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: PANC-1 lysates. ICC/IF: PANC-1 cells. Flow Cyt(Intra): PANC-1 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23858-3 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab259857于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/500.
|
|
ICC/IF |
1/100.
|
|
WB |
1/1000. Predicted molecular weight: 223 kDa.
|
说明 |
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Flow Cyt (Intra)
1/500. |
ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 223 kDa. |
靶标
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功能
May play a role in the development of the nervous system and the pathogenesis of neural tumors. -
组织特异性
Preferentially expressed in total brain, fetal brain, and cerebellum. It is also moderately expressed in the adrenal gland. -
序列相似性
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 26038 Human
- Omim: 610771 Human
- SwissProt: Q8TDI0 Human
- Unigene: 522898 Human
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别名
- ATP-dependent helicase CHD5 antibody
- CHD 5 antibody
- CHD-5 antibody
see all
图片
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All lanes : Anti-CHD5 antibody [EPR23858-3] (ab259857) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 223 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 25247294).
Negative control: HeLa (PMID: 21931736).
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Exposure time: 136 seconds
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Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized PANC-1 cells labelling CHD5 with ab259857 at 1/100 (5.55 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in PANC-1 cell line. Negative control: HeLa (PMID: 29682202) is observed.
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa(Human cervix adenocarcinoma epithelial cell, Left)/ PANC-1 (Human pancreatic epithelioid carcinoma epithelial cell, Right) cells labelling CHD5 with ab259857 at 1/500 dilution (0.1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HeLa (PMID: 29682202).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab259857 尚未被引用在任何文献中。