概述

  • 产品名称
  • 描述
    兔多克隆抗体to CGRP
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic Rat CGRP.

  • 阳性对照
    • Rat colon
  • 常规说明
    Dilute immediately before use with PBS or Tris.

性能

应用

Our Abpromise guarantee covers the use of ab91007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明
    IHC-P: Use at an assay dependent dilution.
    IHC with ABC method

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
    • 细胞定位
      Endoplasmic reticulum and Secreted
    • 数据库链接
    • 别名
      • Calcitonin/calcitonin related polypeptide alpha antibody
      • Alpha type CGRP antibody
      • Beta type CGRP antibody
      • CALC1 antibody
      • CALC2 antibody
      • CALCA antibody
      • CALCB antibody
      • Calcitonin 1 antibody
      • Calcitonin 2 antibody
      • Calcitonin gene related peptide I antibody
      • Calcitonin gene related peptide II antibody
      • Calcitonin related polypeptide alpha antibody
      • Calcitonin related polypeptide beta antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP II antibody
      • CGRP1 antibody
      • CGRP2 antibody
      • CT antibody
      • Katacalcin antibody
      • KC antibody
      see all

    文献

    ab91007 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    1-3 of 3 Q&A

    Question
    Answer

    Thank you for confirming this information and for your help and cooperation with this case.

    As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

    Credit ID: 20223

    As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

    I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

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    Answer

    Thank you for your message and for providing this further information.

    I apologise for any confusion. Information provided in the original questionaire stated the problem was 'non specific staining'. For our investigations, it is important to have correct details on the form. There was also some non specific stianing in some of the images that were kindly sent. Reviewing the images again, it seem the results are inconsistentant. Sometimes there is nonspecific staining, and sometimes no staining.

    In this case, I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or credit note in compensation.

    I look forward to hearing from you with details of how you would like to proceed.

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    Question

    LOT NUMBER GR18150
    DESCRIPTION OF THE PROBLEM Non-specific staining
    SAMPLE Species Human Type of sample: paraffin embedded sections
    PRIMARY ANTIBODY Concentration or dilution:dilute 200X and 50X Diluent buffer : Antibody Diluent, invitrogen,
    LOT 1018726A
    Incubation time: 30 minutes wash Buffer: PBS Number of washes: two times
    DETECTION METHOD HRP Detection
    POSITIVE AND NEGATIVE CONTROLS USED -
    ANTIBODY STORAGE CONDITIONS -20℃
    FIXATION OF SAMPLE Yes/No : yes If yes: Fixative agent and concentration 10% neutral buffered formalin Fixation time : 24 hours Fixation temperature : room temperature
    ANTIGEN RETRIEVAL pH 8.0 Tris-EDTA buffer & pH 6.0 citrate buffer & Proteinase K
    PERMEABILIZATION STEP -
    BLOCKING CONDITIONS not used Endogenous peroxidases blocked? 3% H2O2 room temperature 10 minutes Endogenous biotins blocked? Not used
    SECONDARY ANTIBODY Pollnk-2 Plus HRP Detection kit (Golden Bridge International, Inc.) (Cat No D41-125) Species: Isotype: Reacts against: Mouse & Rabbit Concentration or dilution : Ready-to-use Diluent buffer Incubation time: 15 minutes Fluorochrome or enzyme conjugate: wash Buffer: PBS Number of washes: two times
    HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5
    HAVE YOU RUN A "NO PRIMARY" CONTROL? No
    DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes
    WHAT STEPS HAVE YOU ALTERED? Antibody dilution 200x and 50x Antigen retrieval: HIER(Citrate 6.0, EDTA 8.0), Enzyme(Proteinase K), No treatment endogenous peroxidase blocking time(6 minutes & 10 minutes).
    ADDITIONAL NOTES -

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    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    I would like to reassure you that ab91007 is tested and covered by our 6 month guarantee for use inIHC-P andhuman samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

    Reviewing this case, I would like to offer some suggestions to help optimize the results from ab91007. I would also appreciate if you can confirm some further details:

    1. Please confirm whichtype of tissuewas tested.

    2. I can recommend to include 0.2% Tween in the wash buffer and antibody dilution buffer. Wash 4 times for 5 minutes at each wash step.

    3. I suggest to try reducing the concentration of antibody which should help to reduce the background. Try 1:500.

    4. It woudl be important to include a blocking step. For example, try 5% Serum for 30 minutes. Thismayrequire some optimization.

    5. Is the secondary antibody working well with other primary antibodies? What were the results of the no primary control. The concentration may need to be reduced in order tooptimize the results.

    I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested information and details of how you would like to proceed.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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