The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 12.3 kDa.
Application note: Reducing conditions.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG).
CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity.
Sharma G et al. The interaction of mycobacterial protein Rv2966c with host chromatin is mediated through non-CpG methylation and histone H3/H4 binding. Nucleic Acids Res43:3922-37 (2015).
Read more (PubMed: 25824946) »
Ramsdell TL et al. Linked domain architectures allow for specialization of function in the FtsK/SpoIIIE ATPases of ESX secretion systems. J Mol Biol427:1119-32 (2015).
Read more (PubMed: 24979678) »