概述

  • 产品名称
    Anti-CFP10抗体
  • 描述
    兔多克隆抗体to CFP10
  • 宿主
    Rabbit
  • 特异性
    ab45074 recognises native and E coli expressed recombinant CFP10.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    Reacts with Mycobacterium tuberculosis and Mycobacterium bovis. Not yet tested in other species.
  • 免疫原

    Full length protein (Mycobacterium tuberculosis)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purity: > 95% (by SDS-PAGE)
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab45074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: Use at a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 12.3 kDa.
    Application note: Reducing conditions.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG). CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity.
    • 细胞定位
      Secreted
    • 别名
      • 10 kDa culture filtrate antigen cfp10 antibody
      • Culture filtrate protein 10 antibody
      • ESAT 6 like protein esxB antibody
      • esxB antibody
      • lhp antibody
      • mtsA10 antibody
      • Secreted antigenic protein MTSA 10 antibody
      • tuberculosis CFP10 antibody
      see all

    实验方案

    文献

    This product has been referenced in:
    • Sharma G  et al. The interaction of mycobacterial protein Rv2966c with host chromatin is mediated through non-CpG methylation and histone H3/H4 binding. Nucleic Acids Res 43:3922-37 (2015). WB ; Human . Read more (PubMed: 25824946) »
    • Ramsdell TL  et al. Linked domain architectures allow for specialization of function in the FtsK/SpoIIIE ATPases of ESX secretion systems. J Mol Biol 427:1119-32 (2015). Read more (PubMed: 24979678) »

    See all 2 Publications for this product

    客户评价及客户问答

    Thank you for your interest in Abcam.

    1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding se...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册