概述

  • 产品名称
    Anti-CETP抗体[ATM192]
    参阅全部 CETP 一抗
  • 描述
    小鼠单克隆抗体[ATM192] to CETP
  • 宿主
    Mouse
  • 特异性
    Detects cholesteryl ester transfer protein (CETP) from human and rabbit tissues.
  • 经测试应用
    适用于: ELISA, IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Rabbit, Human
    预测可用于: Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Human CETP aa 149-159.
    Sequence:

    CDSGRVRTDAPD

  • 阳性对照
    • WB,IP: human serum WB: Recombinant Human CETP protein (ab114408), human liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab2726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration. Use protein G beads.
WB 1/400. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF 1/20.

靶标

  • 功能
    Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.
  • 组织特异性
    Expressed by the liver and secreted in plasma.
  • 疾病相关
    Defects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
    Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels.
  • 序列相似性
    Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
  • 细胞定位
    Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BPIFF antibody
    • CETP antibody
    • CETP_HUMAN antibody
    • Cholesteryl ester transfer protein antibody
    • Cholesteryl ester transfer protein plasma antibody
    • HDLCQ10 antibody
    • Lipid transfer protein I antibody
    see all

图片

  • Immunocytochemistry/Immunofluorescence analysis of CETP in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2726 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. CETP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Anti-CETP antibody [ATM192] (ab2726) at 1/500 dilution + Whole cell lysate from rabbit fibroblasts at 40 µg

    Secondary
    HRP-conjugated rabbit anti-mouse at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa


    Exposure time: 2 minutes

    See Abreview

文献

This product has been referenced in:
  • Cho KH  et al. Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double-Blinded, and Placebo-Controlled Study. Oxid Med Cell Longev 2018:4809525 (2018). Read more (PubMed: 29854085) »
  • Park KH  et al. Slim Body Weight Is Highly Associated With Enhanced Lipoprotein Functionality, Higher HDL-C, and Large HDL Particle Size in Young Women. Front Endocrinol (Lausanne) 9:406 (2018). Read more (PubMed: 30072955) »
See all 4 Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (Human THP-1 macrophages)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
70 µg
Treatment
100 µg/ml oxidized LDL (oxLDL) +/- 10 µg/ml apoA-I
Specification
Human THP-1 macrophages
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Anca Sima

Verified customer

提交于 Jun 04 2015

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Sample
Cynomolgus Monkey Serum (serum, PBS diluted)
Specification
serum, PBS diluted
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

提交于 May 30 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Cell lysate - whole cell (Fibroblasts)
Loading amount
40 µg
Specification
Fibroblasts
Treatment
virus
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Oct 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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