Anti-CETP抗体[ATM192] (ab2726)

Reviews (3)Q&A (3)References (4)
Immunocytochemistry/ Immunofluorescence - Anti-CETP antibody [ATM192] (ab2726)

    Key features and details

    • Mouse monoclonal [ATM192] to CETP
    • Suitable for: ICC/IF
    • Reacts with: Rabbit, Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-CETP抗体[ATM192]
      参阅全部 CETP 一抗

    • 描述

      小鼠单克隆抗体[ATM192] to CETP

    • 宿主

      Mouse

    • 特异性

      Detects cholesteryl ester transfer protein (CETP) from human and rabbit tissues.

    • 经测试应用

      适用于: ICC/IFmore details

    • 种属反应性

      与反应: Rabbit, Human
      预测可用于: Cynomolgus monkey

    • 免疫原

      Synthetic peptide corresponding to Human CETP aa 149-159.
      Sequence:

      CDSGRVRTDAPD

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • WB,IP: human serum WB: Recombinant Human CETP protein (ab114408), human liver lysate

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab2726 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    ICC/IF 1/20.

    靶标

    • 功能

      Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.

    • 组织特异性

      Expressed by the liver and secreted in plasma.

    • 疾病相关

      Defects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
      Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels.

    • 序列相似性

      Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

    • 细胞定位

      Secreted > extracellular space.
    • Information by UniProt

    • 数据库链接

      • 别名

        • BPIFF antibody
        • CETP antibody
        • CETP_HUMAN antibody
        • Cholesteryl ester transfer protein antibody
        • Cholesteryl ester transfer protein plasma antibody
        • HDLCQ10 antibody
        • Lipid transfer protein I antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-CETP antibody [ATM192] (ab2726)
        Immunocytochemistry/ Immunofluorescence - Anti-CETP antibody [ATM192] (ab2726)

        Immunocytochemistry/Immunofluorescence analysis of CETP in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2726 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. CETP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      文献 (4)

      发表研究结果有使用 ab2726？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab2726 被引用在 4 文献中.

      • Cho KH  et al. Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double-Blinded, and Placebo-Controlled Study. Oxid Med Cell Longev 2018:4809525 (2018). PubMed: 29854085
      • Park KH  et al. Slim Body Weight Is Highly Associated With Enhanced Lipoprotein Functionality, Higher HDL-C, and Large HDL Particle Size in Young Women. Front Endocrinol (Lausanne) 9:406 (2018). PubMed: 30072955
      • Niculescu LS  et al. Apolipoprotein A-I stimulates cholesteryl ester transfer protein and apolipoprotein E secretion from lipid-loaded macrophages; the role of NF-?B and PKA signaling pathways. Biochem Biophys Res Commun 415:497-502 (2011). WB ; Human . PubMed: 22056562
      • Thomas AP  et al. Mouse monoclonal antipeptide antibodies specific for cholesteryl ester transfer protein (CETP). Hybridoma 15:359-64 (1996). PubMed: 8913785

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      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-CETP antibody [ATM192]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human THP-1 macrophages)
      Gel Running Conditions
      Reduced Denaturing (10% SDS-PAGE)
      Loading amount
      70 µg
      Treatment
      100 µg/ml oxidized LDL (oxLDL) +/- 10 µg/ml apoA-I
      Specification
      Human THP-1 macrophages
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Dr. Anca Sima

      Verified customer

      提交于 Jun 04 2015

      Western blot abreview for Anti-CETP [ATM192] antibody

       Inconclusive
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      15 µg
      Gel Running Conditions
      Reduced Denaturing (4-20% tris-glycine)
      Sample
      Cynomolgus Monkey Serum (serum, PBS diluted)
      Specification
      serum, PBS diluted
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      提交于 May 30 2014

      Western blot abreview for Anti-CETP antibody [ATM192]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rabbit Cell lysate - whole cell (Fibroblasts)
      Loading amount
      40 µg
      Specification
      Fibroblasts
      Treatment
      virus
      Gel Running Conditions
      Reduced Denaturing (gel 10%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      提交于 Oct 02 2008

      Question

      One customer have bought one vial ab2726(CETP antibody ), the lot No is 72205. She used it to test CETP expressed in COS7 cells( transfected with full lenght human or animal CETP gene) at 1:400(recommendated by datasheet). But the result was not good: no bands with short exposure time, then elonged to 15mins, several bands appeared with high backgroud. the detect system is proved to work with other primary antibody. Then, she used another CETP polyclonal antibody prepared by themself to test as contrast. the result is good, clear target bands can be showed( see attchment for result picture) . Can you give me your suggestions to solve this problem asap?

      Read More
      Answer

      I'm sorry to hear your customer is having a problem with ab2726. Could you please tell me what blocking buffer was used (time and temperature too) and what primary incubation buffer was used too (with time and temperature). Has the customer tested the secondary (anti mouse antibody) with other antibodies and on its own? Thank you for your patience, I look forward to hearing from you,

      Read More

      Question

      One customer have bought one vial ab2726(CETP antibody ), the lot No is 72205. She used it to test CETP expressed in COS7 cells( transfected with full lenght human or animal CETP gene) at 1:400(recommendated by datasheet). But the result was not good: no bands with short exposure time, then elonged to 15mins, several bands appeared with high backgroud. the detect system is proved to work with other primary antibody. Then, she used another CETP polyclonal antibody prepared by themself to test as contrast. the result is good, clear target bands can be showed( see attchment for result picture) . Can you give me your suggestions to solve this problem asap?

      Read More
      Answer

      I'm sorry to hear your customer is having a problem with ab2726. Could you please tell me what blocking buffer was used (time and temperature too) and what primary incubation buffer was used too (with time and temperature). Has the customer tested the secondary (anti mouse antibody) with other antibodies and on its own? Thank you for your patience, I look forward to hearing from you,

      Read More

      Question

      Customer would like to know the following regarding ab2726: Is it a kappa or lambda? What is the affinity? Was it used in direct or coating ELISA?

      Read More
      Answer

      The antibody is IgG1. We have not further characterized the antibody in terms of Kappa and Lamda. Here is a refernce for ELISA: A.P. Thomas Mouse Monoclonal Antipeptide Antibodies Specific for Cholesteryl Ester Transfer Protein (CETP). Hybridoma 15(5): 359-364, 1996.

      Read More

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