Anti-Centrin 3抗体(ab228690)
Key features and details
- Rabbit polyclonal to Centrin 3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-Centrin 3抗体 -
描述
兔多克隆抗体to Centrin 3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Rhesus monkey -
免疫原
Recombinant fragment within Human Centrin 3 (internal sequence). The exact sequence is proprietary.
Database link: O15182 -
阳性对照
- ICC/IF: A549 cells. WB: A549, NCI-H1299, HCT 116, C8D30, Neuro-2a, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell extracts. IHC-P: Mouse cervix tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab228690于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 20 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
|
ICC/IF |
1/100 - 1/1000.
|
说明 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 20 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
-
功能
Plays a fundamental role in microtubule-organizing center structure and function. -
序列相似性
Belongs to the centrin family.
Contains 4 EF-hand domains. -
细胞定位
Cytoplasm > cytoskeleton > centrosome. Centrosome of interphase and mitotic cells. - Information by UniProt
-
数据库链接
- Entrez Gene: 1070 Human
- Entrez Gene: 12626 Mouse
- Entrez Gene: 170895 Rat
- Omim: 602907 Human
- SwissProt: O15182 Human
- SwissProt: O35648 Mouse
- Unigene: 591767 Human
- Unigene: 12481 Mouse
-
别名
- CDC31 homolog antibody
- CEN 3 antibody
- CEN3 antibody
see all
图片
-
All lanes : Anti-Centrin 3 antibody (ab228690) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 20 kDa15% SDS-PAGE gel.
-
Paraffin-embedded mouse cervix tissue stained for Centrin 3 using ab228690 at 1/500 dilution in immunohistochemical analysis.
-
All lanes : Anti-Centrin 3 antibody (ab228690) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 7 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 20 kDa15% SDS-PAGE gel.
-
Methanol-fixed A549 (human lung carcinoma cell line) cells stained for Centrin 3 (green) using ab228690 at 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). alpha Tubulin was stained with an anti-alpha Tubulin antibody (red) at 1/500 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab228690 被引用在 1 文献中.
- Verschueren A et al. Planar polarity in primate cone photoreceptors: a potential role in Stiles Crawford effect phototropism. Commun Biol 5:89 (2022). PubMed: 35075261