Anti-Cellubrevin抗体(ab5789)

Relative expression of Cellubrevin observed in Mouse Brain and Rat Brain in comparison to Mouse Liver and Rat Liver using ab5789 to demonstrate specificty of expression of target across different tissues. 

Western blot analysis of recombinant rat VAMP-3 using ab5789.

Western blot analysis of VAMP-3 in HeLa cell extract using ab5789.

Immunofluorescent analysis of formalin-fixed, 0.1% Triton™ X-100 permeabilized, NIH/3T3 (Mouse embryonic fibroblast cell line) cells, blocked with 3% BSA-PBS for 30 minutes at room temperature. Labelling cellubrevin with ab5789 at 1/50 dilution and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody at room temperature in the dark (right image). Compared with negative control without primary antibody (left image). Nuclei were stained with DAPI (Blue). Actin was stained with Alexa Fluor® 555 (Red). The images were captured at 60X magnification.

Immunofluorescent analysis of formalin-fixed, 0.1% Triton™ X-100 permeabilized, Neuro-2a (Mouse neuroblastoma cell line) cells, blocked with 3% BSA-PBS for 30 minutes at room temperature. Labelling cellubrevin with ab5789 at 1/50 dilution and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody at room temperature in the dark (right image). Compared with negative control without primary antibody (left image).  Nuclei were stained with DAPI (Blue).The images were captured at 60X magnification.