Anti-Cellubrevin抗体(ab5789)

Reviews (1)Q&A (7)References (5)
Western blot - Anti-Cellubrevin antibody (ab5789)
  • Western blot - Anti-Cellubrevin antibody (ab5789)

Key features and details

  • Rabbit polyclonal to Cellubrevin
  • Suitable for: WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

概述

  • 产品名称

    Anti-Cellubrevin抗体
    参阅全部 Cellubrevin 一抗

  • 描述

    兔多克隆抗体to Cellubrevin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Human, Recombinant fragment
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Mouse Cellubrevin aa 1-18.
    Sequence:

    MSTGVPSGSSAATGSNRR


    (Peptide available as ab5861)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • In Western Blot HeLa cell extract (ab7898) can be used as a positive control.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • Primary antibody说明

    The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, all VAMP isoform levels appear to be significantly reduced.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5789于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
说明
WB
1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).

靶标

图片

  • Western blot - Anti-Cellubrevin antibody (ab5789)
    Western blot - Anti-Cellubrevin antibody (ab5789)

    Western blot analysis of recombinant rat VAMP-3 using ab5789.

  • Western blot - Anti-Cellubrevin antibody (ab5789)
    Western blot - Anti-Cellubrevin antibody (ab5789)

    Western blot analysis of VAMP-3 in HeLa cell extract using ab5789.

文献 (5)

发表研究结果有使用 ab5789？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5789 被引用在 5 文献中.

  • Lasic E  et al. Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action. Sci Rep 9:10957 (2019). PubMed: 31358895
  • Habtemichael EN  et al. Usp25m protease regulates ubiquitin-like processing of TUG proteins to control GLUT4 glucose transporter translocation in adipocytes. J Biol Chem 293:10466-10486 (2018). PubMed: 29773651
  • Verboogen DRJ  et al. Secretory vesicles of immune cells contain only a limited number of interleukin 6 molecules. FEBS Lett 592:1535-1544 (2018). PubMed: 29570778
  • Verboogen DRJ  et al. Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. Elife 6:N/A (2017). WB, ICC/IF ; Human . PubMed: 28524818
  • Caceres PS  et al. Vesicle-associated membrane protein 2 (VAMP2) but Not VAMP3 mediates cAMP-stimulated trafficking of the renal Na+-K+-2Cl- co-transporter NKCC2 in thick ascending limbs. J Biol Chem 289:23951-62 (2014). WB ; Rat . PubMed: 25008321

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Western blot abreview for Anti-Cellubrevin antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (4-20 % gradient gel)
Sample
Human Cell lysate - whole cell (HeLa, U266)
Specification
HeLa, U266
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 13 2013

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