重组Anti-CEBP Alpha/CEBPA抗体[EP709Y] - BSA and Azide free (ab219726)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP709Y] to CEBP Alpha/CEBPA - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CEBP Alpha/CEBPA抗体[EP709Y] - BSA and Azide free
参阅全部 CEBP Alpha/CEBPA 一抗 -
描述
兔单克隆抗体[EP709Y] to CEBP Alpha/CEBPA - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- U937 cell lysate
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常规说明
ab219726 is the carrier-free version of ab40764.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP709Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab219726于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
靶标
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功能
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. -
序列相似性
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1050 Human
- Entrez Gene: 12606 Mouse
- Entrez Gene: 24252 Rat
- Omim: 116897 Human
- SwissProt: P49715 Human
- SwissProt: P53566 Mouse
- SwissProt: P05554 Rat
- Unigene: 76171 Human
see all -
别名
- Apoptotic cysteine protease antibody
- Apoptotic protease Mch 5 antibody
- C/EBP alpha antibody
see all
图片
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All lanes : Anti-CEBP Alpha/CEBPA antibody [EP709Y] (ab40764) at 1/1000 dilution (Purified)
Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDaThis data was developed using ab40764, the same antibody clone in a different buffer formulation.
This antibody detects high backgrounds.
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (5)
ab219726 被引用在 5 文献中.
- Weston W et al. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets. Sci Rep 4:5199 (2014). WB ; Mouse . PubMed: 24903657
- Marzook H et al. Metastasis-associated protein 1 drives tumor cell migration and invasion through transcriptional repression of RING finger protein 144A. J Biol Chem 287:5615-26 (2012). WB, IP, EMSA ; Human . PubMed: 22184113
- Kilroy G et al. The ubiquitin ligase Siah2 regulates PPAR? activity in adipocytes. Endocrinology 153:1206-18 (2012). PubMed: 22294748
- Bristol JA et al. The Epstein-Barr virus BZLF1 protein inhibits tumor necrosis factor receptor 1 expression through effects on cellular C/EBP proteins. J Virol 84:12362-74 (2010). PubMed: 20861254
- Bristol JA et al. CCAAT/enhancer binding proteins alpha and beta regulate the tumor necrosis factor receptor 1 gene promoter. Mol Immunol 46:2706-13 (2009). PubMed: 19523687