Key features and details
- Rabbit polyclonal to CDYL
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
参阅全部 CDYL 一抗
经测试应用适用于: WBmore details
种属反应性与反应: Human, African green monkey
预测可用于: Mouse, Rat
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
- This antibody gave a positive signal in COS7 cells transfected with Human CDYL (Whole Cell Lysate)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
功能Acts as repressor of transcription (By similarity). Has histone acetyltransferase activity, with a preference for histone H4.
组织特异性Ubiquitous. Expressed at moderate levels in all tissues examined.
序列相似性Contains 1 chromo domain.
- Information by UniProt
- CDY-like antibody
- CDY-like autosomal antibody
- Cdyl antibody
All lanes : Anti-CDYL antibody (ab5188) at 1 µg/ml
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate - Untransfected
Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
All lanes : Anti-CDYL antibody (ab5188) at 1/1000 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid
Predicted band size: 66 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
Rabbit polyclonal to CDYL (ab5188) at 1/1000.
Left lane (-) COS7 (monkey) cells.
Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.
ab5188 被引用在 11 文献中.
- Pham V et al. Proteomic Analyses Identify a Novel Role for EZH2 in the Initiation of Cancer Cell Drug Tolerance. J Proteome Res 19:1533-1547 (2020). PubMed: 32159963
- Qiu Z et al. CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics 9:4717-4729 (2019). PubMed: 31367252
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
- Qi C et al. Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2. J Neurosci 34:4494-508 (2014). Rat . PubMed: 24671995
- Escamilla-Del-Arenal M et al. Cdyl, a new partner of the inactive X chromosome and potential reader of H3K27me3 and H3K9me2. Mol Cell Biol 33:5005-20 (2013). WB, ICC/IF . PubMed: 24144980