HRP Anti-CDKN2A/p19ARF抗体[5-C3] (ab202225)
Key features and details
- HRP Rat monoclonal [5-C3] to CDKN2A/p19ARF
- Suitable for: WB
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
HRP Anti-CDKN2A/p19ARF抗体[5-C3]
参阅全部 CDKN2A/p19ARF 一抗 -
描述
HRP大鼠单克隆抗体[5-C3] to CDKN2A/p19ARF -
宿主
Rat -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
不与反应: Hamster, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: MEF-1 whole cell lystate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Affinity purified -
克隆
单克隆 -
克隆编号
5-C3 -
骨髓瘤
Y3/Ag1.2.3 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab202225于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
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说明 |
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WB
1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). |
靶标
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相关性
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 12578 Mouse
- SwissProt: Q64364 Mouse
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别名
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
see all
图片
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HRP Anti-CDKN2A/p19ARF antibody [5-C3] (ab202225) at 1/5000 dilution + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202225 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab202225 被引用在 4 文献中.
- Miao J et al. Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells. Physiol Rep 9:e14696 (2021). PubMed: 33463897
- Gong W et al. Brahma-related gene-1 promotes tubular senescence and renal fibrosis through Wnt/ß-catenin/autophagy axis. Clin Sci (Lond) 135:1873-1895 (2021). PubMed: 34318888
- Miao J et al. Wnt/ß-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction. Aging Cell 18:e13004 (2019). PubMed: 31318148
- Luo C et al. Wnt9a Promotes Renal Fibrosis by Accelerating Cellular Senescence in Tubular Epithelial Cells. J Am Soc Nephrol 29:1238-1256 (2018). PubMed: 29440280