Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
50 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: NA931 GE healthcare
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Amersham ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 19 kDa Non-specific: 25, 35 kDa
Additional data
Additional Notes
Samples used: mouse kidney tissue
Lane 2,4,6,8,10,12 - Transgenic mice expressing BRAFV600E specifically in the kidney.
Lane 3,5,7,9,11,13 - Littermate mice (normal)
BRAF V600E overexpression induces oncogene-induced senescence.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Archana Raman
Verified customer
提交于 Dec 08 2017