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RabMAb

Anti-CDKN2A/p16INK4a抗体[EPR20418] (ab211542)

概述

  • 产品名称
    Anti-CDKN2A/p16INK4a抗体[EPR20418]
    参阅全部 CDKN2A/p16INK4a 一抗
  • 描述
    兔单克隆抗体[EPR20418] to CDKN2A/p16INK4a
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant full length protein within Mouse CDKN2A/p16INK4a aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P51480

  • 阳性对照
    • WB: MEF whole cell lysate; His-tagged mouse CDKN2A/p16INK4a recombinant protein, aa1-168. ICC/IF: MEF cells. Flow Cyt: MEF cells. IP: MEF whole cell lysate.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab211542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 16, 14 kDa (predicted molecular weight: 18 kDa).
IP 1/30.
ICC/IF 1/100.
Flow Cyt 1/500.

靶标

  • 细胞定位
    Cytoplasmic and Nuclear
  • 数据库链接
  • 形式
    There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
  • 别名
    • CCM2 antibody
    • CDK4 inhibitor p16 INK4 antibody
    • CDK4I antibody
    • CDKN2 antibody
    • CDKN2A antibody
    • Cell cycle negative regulator beta antibody
    • CMM2 antibody
    • Cyclin dependent kinase 4 inhibitor A antibody
    • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
    • Cyclin Dependent Kinase Inhibitor 2A antibody
    • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
    • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
    • Cyclin dependent kinase inhibitor p16 antibody
    • INK4 antibody
    • INK4A antibody
    • MLM antibody
    • MTS1 antibody
    • Multiple tumor suppressor 1 antibody
    • p14 antibody
    • p16 antibody
    • P16INK4 antibody
    • p16INK4a antibody
    • p19 antibody
    • p19Arf antibody
    • TP16 antibody
    see all

图片

  • Immunofluorescent analysis of 100% methanol-fixed MEF (mouse embryonic fibroblast cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling CDKN2A/p16INK4a with ab211542  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on MEF cell line.

    Negative control: NIH/3T3 (PMID: 15210712).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution.

    MEF cells were kindly provided by professor Pinlong Xu, Zhejiang University.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed MEF (mouse embryonic fibroblast cell line) cell line labeling CDKN2A/p16INK4a with ab211542 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    MEF cells were kindly provided by professor Pinlong Xu, Zhejiang University.

  • All lanes : Anti-CDKN2A/p16INK4a antibody [EPR20418] (ab211542) at 1/1000 dilution

    Lane 1 : His-tagged mouse CDKN2A/p16INK4a recombinant protein
    Lane 2 : His-tagged mouse CDKN2B/p15INK4b recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-CDKN2A/p16INK4a antibody [EPR20418] (ab211542) at 1/2000 dilution

    Lane 1 : MEF (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 3 : Mouse lung lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Mouse testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 18 kDa
    Observed band size: 14,16 kDa (why is the actual band size different from the predicted?)


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: NIH/3T3 (PMID: 15210712).

    Limited expression in mouse normal tissues has been documented in the literature (PMID: 9244355).

    14 kDa band is a proteolytic p16INK4a lacking C-terminus (PMID: 18053084).

    MEF cells are kindly provided by professor Pinlong Xu, Zhejiang University.

  • CDKN2A/p16INK4a was immunoprecipitated from 0.35 mg of MEF (mouse embryonic fibroblast cell line) whole cell lysate with ab211542 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211542 at 1/5000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: MEF whole cell lysate 10 μg (Input). 

    Lane 2: ab211542 IP in MEF whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211542 in MEF whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    MEF cells were kindly provided by professor Pinlong Xu, Zhejiang University.

文献

This product has been referenced in:
  • Zhang X  et al. High-fat diet modifies expression of hepatic cellular senescence gene p16(INK4a) through chromatin modifications in adult male rats. Genes Nutr 13:6 (2018). Read more (PubMed: 29564021) »

See 1 Publication for this product

客户评价及客户问答

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Senescent cells in 18 month old arthritic mouse kn)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Senescent cells in 18 month old arthritic mouse kn
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Fixative
formalin
Username

Dr. Matthew Wolf

Verified customer

提交于 Jun 06 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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