Anti-CDKN2A/p16INK4a抗体[DCS50.1] (ab16123)

概述

  • 产品名称
    Anti-CDKN2A/p16INK4a抗体[DCS50.1]
    参阅全部 CDKN2A/p16INK4a 一抗
  • 描述
    小鼠单克隆抗体[DCS50.1] to CDKN2A/p16INK4a
  • 宿主
    Mouse
  • 特异性
    This antibody shows no cross reactivity with the closely related inhibitors p15INK4b and p18INK4c.
  • 经测试应用
    适用于: ICC/IF, IHC-P, WB, IP, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human CDKN2A/p16INK4a aa 1-156.
    Sequence:

    MEPAAGSSMEPSADWLATAAARGRVEEVRALLEAGALPNAPNSYGRRPIQ VMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRA GARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEG PSDIPD


    Database link: P42771

  • 阳性对照
    • WB: HEK293 and HeLa cell lysates. Flow cyt: HEK293 cells. IHC-P: Bladder tumor tissue.

性能

应用

Our Abpromise guarantee covers the use of ab16123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IP Use at an assay dependent concentration.

ab16123 co-precipitates cdk4/cdk6.

Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 细胞定位
    Cytoplasmic and Nuclear
  • 数据库链接
  • 形式
    There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
  • 别名
    • CCM2 antibody
    • CDK4 inhibitor p16 INK4 antibody
    • CDK4I antibody
    • CDKN2 antibody
    • CDKN2A antibody
    • Cell cycle negative regulator beta antibody
    • CMM2 antibody
    • Cyclin dependent kinase 4 inhibitor A antibody
    • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
    • Cyclin Dependent Kinase Inhibitor 2A antibody
    • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
    • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
    • Cyclin dependent kinase inhibitor p16 antibody
    • INK4 antibody
    • INK4A antibody
    • MLM antibody
    • MTS1 antibody
    • Multiple tumor suppressor 1 antibody
    • p14 antibody
    • p16 antibody
    • P16INK4 antibody
    • p16INK4a antibody
    • p19 antibody
    • p19Arf antibody
    • TP16 antibody
    see all

图片

  • All lanes : Anti-CDKN2A/p16INK4a antibody [DCS50.1] (ab16123) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa
    Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded bladded tumor tissue labelling CDKN2A/p16INK4a with ab16123 at 10µg/ml.

  • ICC/IF image of ab16123 stained Hepp cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) ab150113) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HEK293 cells stained with ab16123 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16123, 1µg/1x106 cells ) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

文献

This product has been referenced in:
  • Kannappan R  et al. p53 Modulates the Fate of Cardiac Progenitor Cells Ex Vivo and in the Diabetic Heart In Vivo. EBioMedicine 16:224-237 (2017). Read more (PubMed: 28163043) »
  • Fernández-Torrón R  et al. Cancer risk in DM1 is sex-related and linked to miRNA-200/141 downregulation. Neurology 87:1250-7 (2016). Read more (PubMed: 27558368) »

See all 4 Publications for this product

客户评价及客户问答

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Application
ELISA
Sample
Human Recombinant protein (Purified recombinant p16 (overexpressed in HEK293))
Specification
Purified recombinant p16 (overexpressed in HEK293)
Type
Sandwich (Capture)
Blocking step
SuperBlock PBS Pierce #37580 as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10µg/mL
Username

Abcam user community

Verified customer

提交于 Apr 28 2014

Application
Western blot
Sample
Cow Cell lysate - whole cell (vascular endothelial cells)
Loading amount
20 µg
Specification
vascular endothelial cells
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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