Anti-CDKN2A/p14ARF抗体[ARF 4C6/4] (ab11048)
Key features and details
- Mouse monoclonal [ARF 4C6/4] to CDKN2A/p14ARF
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-CDKN2A/p14ARF抗体[ARF 4C6/4]
参阅全部 CDKN2A/p14ARF 一抗 -
描述
小鼠单克隆抗体[ARF 4C6/4] to CDKN2A/p14ARF -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Bacteria CDKN2A/p14ARF.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
ARF 4C6/4 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11048于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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相关性
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. -
细胞定位
Cytoplasmic and Nuclear -
数据库链接
- Entrez Gene: 1029 Human
- Omim: 600160 Human
- SwissProt: P42771 Human
- SwissProt: Q8N726 Human
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别名
- ARF antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
see all
图片
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ICC/IF image of ab11048 stained human MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11048, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2cells.
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Overlay histogram showing HeLa cells stained with ab11048 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11048, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab11048 被引用在 6 文献中.
- Lee CK et al. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene 35:1988-95 (2016). PubMed: 26212014
- Hamilton G et al. AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget 5:6142-67 (2014). PubMed: 25071014
- Jeanblanc M et al. Parallel pathways in RAF-induced senescence and conditions for its reversion. Oncogene 31:3072-85 (2012). PubMed: 22020327
- Ma H & Pederson T Depletion of the nucleolar protein nucleostemin causes G1 cell cycle arrest via the p53 pathway. Mol Biol Cell 18:2630-5 (2007). WB, ICC/IF ; Human . PubMed: 17494866
- Clark PA et al. Multiple interacting domains contribute to p14ARF mediated inhibition of MDM2. Oncogene 21:4498-507 (2002). PubMed: 12085228
- Llanos S et al. Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus. Nat Cell Biol 3:445-52 (2001). PubMed: 11331871